四味镇痛中药对内腓肽降解酶作用的实验研究

目的 :研究四味镇痛中药对内腓肽降解酶的作用。方法 :从大鼠的肾脏制备含有内腓肽降解酶的物质 ,建立内腓肽降解酶活性检测模型 ;并用此模型观察四味镇痛中药提取物对内腓肽降解酶特别是对中性内肽酶活性 (NEP2 4 .11)的抑制作用。结果 :(1)钩藤和羌活的水提物显示很强的NEP2 4 11酶抑制作用 ;(2 )无论是有机提取物还是水提物 ,延胡索和川芎对NEP2 4 11的抑制作用都弱于钩藤和羌活 ;(3)钩藤和羌活不仅是氨肽酶和中性内肽酶的双重抑制剂 ,而且是氨肽酶、中性内肽酶和血管紧张素转化酶的三重抑制剂。结论 :四味镇痛中药具有不同的镇痛机制 ,其中 ,钩藤和羌活通过抑制氨肽酶和中性内肽酶而发挥镇痛作用

THE EXPERIMENTAL RESEARCH FOR THE EFFECTS OF FOUR CHINESE HERBAL EXTRACT ON ENKEPHALIN-HYDROLYSING ENZYMES

AIM:To study the effects of four Chinese herbal extracts on enkephalin hydrolysing enzymes.METHODS:The crude microsomal fraction containing enkephalin hydrolysing enzymes was prepared from the rat kidney and the enkephalin hydrolysing enzymes assay was established.Then the inhibitory effects of four Chinese herbal extracts on enkephalin hydrolysing enzymes were observed in vitro. RESULTS:The experiments showed that the aqueous extract of Gou teng(Uncaaria rhynchophylia Jacks.)and Qiang huo(Angelica grosserrata Maxim.)have significant inhibitory effects on NEP but results with organic solvent extracts were not significant.Both aqueous and organic extracts of Yan husu(Corydalis ambiguach.Etschl.)and Chuan xiong(Conioselinum unvittatum Turcz)showed less inhibitory activity than that of Gou teng and Qiang huo.The experiments showed that Gou teng water extract(Gwext)and Qianghuo water extract(Qwext)were not only dual inhibitors but also triple inhibitors of NEP/APN/ACE.CONCLUSION:This suggested that the four herbs may have different bases for their analgesic activity.Gou teng and Qiang huo have the analgesic activity by way of inhibiting APN and NEP24.11.