| 豆皮水溶性多糖组分SHP-3的物化性质研究(英文) |
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| 引用本文： | 赖富饶,吴晖,李晓凤,任尧,吴磊燕,温其标.豆皮水溶性多糖组分SHP-3的物化性质研究(英文)[J].天然产物研究与开发,2011,23(1):76-81. |
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| 作者姓名： | 赖富饶 吴晖 李晓凤 任尧 吴磊燕 温其标 |
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| 作者单位： | 1. 华南理工大学轻工与食品学院,广州,510640
2. 华南理工大学轻工与食品学院,广州,510640;江西农业大学食品科学与工程学院,南昌,330045 |
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| 基金项目： | supported by Program for New Century Excellent Talents in University(NCET-06-0746) |
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| 摘 要： | 对豆皮采用热水浸提得到豆皮水溶性多糖,通过DEAE-cellulose离子交换柱洗脱,得到3个组分。本文主要研究了采用0.3 M NaOH溶液洗脱纯化得到的组分SHP-3的物化性质。对SHP-3进行凝胶渗透色谱、气相色谱分析、紫外光谱及高碘酸氧化-Smith降解分析,结果表明:SHP-3的分子量为45554,其糖醛酸含量为26.71%(wt.%),单糖组成摩尔比为Rah:Fuc:Ara:Xyl:Man:Gal:Glu=3.55:0.44:11.58:1:7.45:5.12:1.12,紫外光谱在260～280 nm没有吸收峰,表明没有蛋白质或核酸等物质;高碘酸氧化-Smith降解结果表明SHP-3的连接结构以为(1→4)糖苷键为主,其摩尔比例占68.9%,(1→2)糖苷键占11.4%,(1→6)糖苷键占19.7%。
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| 关 键 词： | 豆皮 水溶性多糖 纯化 单糖组成 气相色谱 |
Physicochemical Properties of a Water-soluble Polysaccharide Fraction SHP-3 Purified from Soybean Hull |
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| LAI Fu-rao,WU Hui,LI Xiao-feng,REN Yao,WU Lei-yan,WEN Qi-biao.Physicochemical Properties of a Water-soluble Polysaccharide Fraction SHP-3 Purified from Soybean Hull[J].Natural Product Research and Development,2011,23(1):76-81. |
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| Authors: | LAI Fu-rao WU Hui LI Xiao-feng REN Yao WU Lei-yan WEN Qi-biao |
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| Institution: | LAI Fu-rao1,WU Hui1,LI Xiao-feng1,REN Yao1,WU Lei-yan1,2,WEN Qi-biao11College of Light Industry and Food Sciences,South China University of Technology,Guangzhou 510640,China,2College of Food Science and Engineering,Jiangxi Agricultural University,Nanchang 330045 |
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| Abstract: | The water-soluble polysaccharide of soybean hull (SHP) was extracted by hot water and three fractions were obtained by using DEAE-cellulose anion exchange column.The physicochemical properties of the purified fractions SHP3,eluted with 0.3 M NaOH,were investigated.Gel permeation chromatography showed that the molecule weight of SHPviolet spectra indicated that there was no presence of protein in SHP-3.The molar percentages of (1→2) glycosidic linkage,(1→4) glycosidic linkage and (1→6) glycosidic linkage were 11.4%,68.9% and 19.7%,respectively. |
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| Keywords: | soybean hull water-soluble polysaccharide purification monosaccharide composition GC |
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