| 一种蚯蚓体腔液蛋白的分离纯化及其生物活性 |
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| 引用本文： | 张阳,王艳红,张华,秦学功,崔玉东.一种蚯蚓体腔液蛋白的分离纯化及其生物活性[J].天然产物研究与开发,2011,23(1):89-93. |
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| 作者姓名： | 张阳 王艳红 张华 秦学功 崔玉东 |
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| 作者单位： | 黑龙江八一农垦大学生命科学技术学院,大庆,163319 |
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| 基金项目： | 黑龙江八一农垦大学科研启动基金 |
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| 摘 要： | 赤子爱胜蚓体腔液含有多种蛋白并具有广泛的生物学活性,本研究采用6 V电压刺激蚯蚓分泌体腔液,通过离心、超滤分离得到蚯蚓体腔液蛋白粗品;然后将蛋白粗品经过凝胶层析和离子交换层析纯化,得到一种蚯蚓体腔液蛋白。以离子交换色谱分离到的蚯蚓体腔液蛋白样品为受试样品,紫外光谱扫描显示该蛋白在280nm处有最大吸收峰,SDS-PAGE凝胶电泳测定其分子量约为38.6 KDa,比浊抑菌试验结果显示低浓度受试样品作用大肠杆菌1 h出现抑制效果,作用金黄色葡萄球菌3h出现抑制效果,表明该样品具有一定抑制细菌生长的活性,此外,该蛋白还具有较强的溶解鸡红细胞的作用,溶解红细胞的最低浓度为0.39μg/mL,提示此蛋白的生物活性可能是通过破环细胞结构而实现的。
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| 关 键 词： | 蚯蚓 体腔液 分离纯化 生物活性 |
Purification of Earthworm Protein from Coelomic Fluid and Its Bioactivity |
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| ZHANG Yang,WANG Yan-hong,ZHANG Hua,QIN Xue-gong,CUI Yu-dong.Purification of Earthworm Protein from Coelomic Fluid and Its Bioactivity[J].Natural Product Research and Development,2011,23(1):89-93. |
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| Authors: | ZHANG Yang WANG Yan-hong ZHANG Hua QIN Xue-gong CUI Yu-dong |
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| Institution: | ZHANG Yang,WANG Yan-hong,ZHANG Hua,QIN Xue-gong,CUI Yu-dong College of Biological Science and Technology,Heilongjiang Bayi Agricultural University,Daqing 163319,China |
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| Abstract: | The coelomic fluid of earthworm Eisenia foetida exhibit extensively biological activity previously.Earthworm was induced by 6-Voltages electric stimulation to secrete coelomic fluid,followed by centrifugation and ultrafiltration to obtain a crude protein mixture.The crude extracts have been purified by means of gel chromatography and anion-exchange chromatography in sequence,as resulted in obtaining a kind of pure protein.Ultraviolet analysis indicated the protein absorption wavelength 280 nm,and SDS-PAGE d... |
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| Keywords: | earthworm coelomic fluid purification biological activity |
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