| 高效液相色谱法测定山楂叶中熊果酸含量的研究 |
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| 引用本文： | 任凤莲,吴梅林,谷芳芳.高效液相色谱法测定山楂叶中熊果酸含量的研究[J].天然产物研究与开发,2007,19(2):303-305. |
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| 作者姓名： | 任凤莲 吴梅林 谷芳芳 |
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| 作者单位： | 1. 中南大学化学化工学院,长沙,410083
2. 中南大学化学化工学院,长沙,410083;中国科学院南海海洋研究所,广州,510301 |
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| 基金项目： | This work was supported by the Committee of Science and Technology of Xiangxi Miao and Tujia nationalities, Hunan, China. |
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| 摘 要： | 本文建立了一种可靠性高、重现性好的高效液相色谱(HPLC)测定山楂叶中熊果酸含量的方法,在测定中采用富集和固相萃取组合纯化工艺去除干扰物质。高效液相色谱测定条件为Hypersil(ODS)色谱柱,流动相为甲醇:0.2%磷酸二氢钠(90∶10,V/V),检测波长210nm,流速0.8mL/min。熊果酸浓度在100~800μg/mL与峰面积存在良好线性关系(r2=0.9992),该方法准确可靠,日内稳定性标准偏差在0.6%~1.5%,日间稳定性标准偏差在0.7%~2.6%。为不同产地山楂叶中熊果酸含量建立有效的分析方法。
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| 关 键 词： | 山楂叶 熊果酸 纯化工艺 |
| 文章编号： | 1001-6880（2007）02-0303-03 |
| 收稿时间： | 2005-06-27 |
| 修稿时间： | 2005-11-22 |
Determination of Ursolic Acid in Hawthorn Leaves( Crateagus pinnatifida) by HPLC |
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| REN Feng-lian,WU Mei-lin,GU Fang-fang.Determination of Ursolic Acid in Hawthorn Leaves( Crateagus pinnatifida) by HPLC[J].Natural Product Research and Development,2007,19(2):303-305. |
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| Authors: | REN Feng-lian WU Mei-lin GU Fang-fang |
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| Institution: | 1.institute of Instrumental Analysis, College of Chemistry and Chemical Engineering Central South University, Changsha 410083, China ;2. South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China |
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| Abstract: | Quantitative HPLC analysis of ursolic acid in leaves of Hawthorn was established. The HPLC system used an Hypersil(ODS) column eluted with methanol:0.2% sodium dihydrogen phosphate aqueous = 90: 10,V/V) as solvents at a flow rate 0.8 mL/min and detected at 210 nm. Linear correlation ( r2 =0.9992) of the peak area and the concentration of ursolic acid over the concentration range 100 ～ 800 μg/mL was obtained. The method was precise with relative standard deviations for ursolic acid ranged between 0.6% ～ 1.5 % ( intraday ) and 0.7% ～ 2.6% ( interday ). The content of ursolic acid in the leaves of Hawthorn growing at different areas was determined to establish the effectiveness of the method. |
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| Keywords: | HPLC Hawthorn leaves ursolic acid purification technology HPLC |
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