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| 高速逆流色谱法分离制备丹酚酸B | |
| 引用本文： | 王凤美,陈军辉,李磊,黎先春,王小如.高速逆流色谱法分离制备丹酚酸B[J].天然产物研究与开发,2006,18(1):100-104. |
| 作者姓名： | 王凤美 陈军辉 李磊 黎先春 王小如 |
| 作者单位： | 1. 厦门大学化学化工学院化学系现代分析科学教育部重点实验室,厦门,361005 2. 南昌大学食品科学教育部重点实验室,南昌,330047 3. 国家海洋局第一海洋研究所青岛市现代分析科技与中药标准化重点实验室,青岛266061 4. 厦门大学化学化工学院化学系现代分析科学教育部重点实验室,厦门,361005;国家海洋局第一海洋研究所青岛市现代分析科技与中药标准化重点实验室,青岛266061 |
| 基金项目： | 国家自然科学基金重点项目(20235020)，厦门市科技创新重点项目(3502Z20031096) |
| 摘 要： | 采用高速逆流色谱法分离纯化丹参水溶性成分丹酚酸类物质,制备丹酚酸B化学对照品。分离采用的溶剂系统为正己烷-乙酸乙酯-水-甲醇(1.5:5:5:1.5),上相做固定相,下相做流动相,流速为1.7 mL/min,仪器转速850 rpm,进样量80 mg,纯度用HPLC方法测定。结果表明:一次分离可制备63.4 mg丹酚酸B,其纯度为98.6%。该方法操作简单,可作为高纯度丹酚酸B化学对照品的制备分离方法。 |
| 关 键 词： | 丹参 丹酚酸B 高速逆流色谱 |
| 文章编号： | 1001-6880（2006）01-0100-05 |
| 收稿时间： | 2005-05-27 |
| 修稿时间： | 2005-06-21 |
Preparative Separation of Salvianolic Acid B from Salvia miltiorrhiza by High Speed Counter-Current Chromatorgraphy |
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| WANG Feng-mei,CHEN Jun-hui,LI Lei,Li Sen-Chun,WANG Xiao-ru.Preparative Separation of Salvianolic Acid B from Salvia miltiorrhiza by High Speed Counter-Current Chromatorgraphy[J].Natural Product Research and Development,2006,18(1):100-104. | |
| Authors: | WANG Feng-mei CHEN Jun-hui LI Lei Li Sen-Chun WANG Xiao-ru |
| Institution: | 1. The Key laboratory of Analytical Science of MOE, the College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China;2. The Key laboratory of Food Science of Ministry of Education, Nanchang University,Nanchang 330047, China;3. Qingdao Key tab on Analytical Technology Development and Standardization of Chinese Medicines, First Institute Oceanography of SOA, Qingdao 266061, China |
| Abstract: | Salvianolic acid B,a major bioactive component in Danshen(Salvia miltiorrhiza Bge.),was isolated and purified by high-speed counter-current chromatography(HSCCC) in semi-preparative scale from the crude extract of Danshen.Separation was performed with a two-phase solvent system composed of hexane-ethyl acetate-water-methanol(1.5:5:5:1.5,v/v) by eluting the lower aqueous phase at a flow-rate of 1.7 mL/min and a revolution speed of 850 rpm with injection volume of 80 mg.After the proceeding of separation,63.4 mg salvianolic acid B was obtained,and analyzed by normalization method according to HPLC areas giving a purity of 98.6%.HSCCC is a recommendable method to prepare and purify the active constituents from Salvia miltiorrhiza. |
| Keywords: | Salvia mltiorrhiza Salvianolic acid B HSCCC |
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