| 链霉菌FIM-080014产生的核苷类代谢产物研究 |
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| 引用本文： | 王传喜,彭飞,江宏磊,江宁宇,陈名洪,林如,魏莹,江红,连云阳.链霉菌FIM-080014产生的核苷类代谢产物研究[J].天然产物研究与开发,2012,24(2):143-146. |
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| 作者姓名： | 王传喜 彭飞 江宏磊 江宁宇 陈名洪 林如 魏莹 江红 连云阳 |
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| 作者单位： | 福建省微生物研究所,福建省新药(微生物)筛选重点实验室,福州350007 |
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| 基金项目： | 福建省自然科学基金项目(2010501081);重大新药创制(2010ZX09401-403);福建省微生物研究所所立项目(FIMZ2011006) |
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| 摘 要： | 本文采用大孔吸附树脂、硅胶柱色谱、反相柱色谱、凝胶sephadex LH-20及HPLC等方法对链霉菌FIM-080014发酵液及菌丝体中的代谢产物进行分离,得到7个核苷类化合物。通过NMR及MS等方法鉴定了上述化合物的结构,分别为尿嘧啶(1)、尿嘧啶核苷(2)、2'-脱氧尿嘧啶核苷(3)、5'-C-甲基尿嘧啶核苷(4)、2'-脱氧胸腺嘧啶核苷(5)、2'-脱氧鸟嘌呤核苷(6)和次黄嘌呤(7),其中化合物4首次从微生物代谢产物中分离得到。活性分析表明化合物1～7对神经氨酸酶具有一定的抑制活性,其IC50值分别为3.7、2.1、4.4、1.4、3.6、2.5和2.4 mM。
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| 关 键 词： | 链霉菌 代谢产物 核苷 神经氨酸酶活性 |
Nucleoside Metabolites Produced by Streptomyces FIM-080014 |
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| WANG Chuan-xi
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PENG Fei
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JIANG Hong-lei
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JIANG Ning-yu
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CHEN Ming-hong
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LIN Ru
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WEI Ying
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JIANG Hong
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LIAN Yun-yang.Nucleoside Metabolites Produced by Streptomyces FIM-080014[J].Natural Product Research and Development,2012,24(2):143-146. |
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| Authors: | WANG Chuan-xi PENG Fei JIANG Hong-lei JIANG Ning-yu CHEN Ming-hong LIN Ru WEI Ying JIANG Hong LIAN Yun-yang |
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| Institution: | Fujian Key Laboratory of Screening for Novel Microbial Products,Fujian Instutite of Microbiology,Fuzhou 350007,China |
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| Abstract: | Seven nucleoside metabolites were isolated from the fermentation broth of Streptomyces sp.FIM-080014 by using HP-20 macroporous adsorption resin,silica gel column chromatography,ODS column chromatography,sephadex LH-20 and semi-preparative HPLC.They were identified as uracil(1),uridine(2),2′-deoxyuridine(3),5′-C-methyluridine(4), 2′-deoxythymidine(5),2′-deoxyguanosine(6) and hypoxanthine(7) based on the data of NMR and MS.Bioactive assay revealed that compounds 1-7 showed slight antineuraminidase activity,with IC50 values of 3.7,2.1,4.4,1.4,3.6,2.5 and 2.4 mM,respectively.In addition,compound 4 was isolated from microbial metabolites for the first time. |
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| Keywords: | Streptomyces metabolites nucleoside neuraminidase activity |
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