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江浙蝮蛇蛇毒蛋白诱导K562细胞调亡的研究
引用本文:王春玲,曹小红.江浙蝮蛇蛇毒蛋白诱导K562细胞调亡的研究[J].天然产物研究与开发,2004,16(5):387-390.
作者姓名:王春玲  曹小红
作者单位:天津科技大学食品科学与生物工程学院 天津 300222
摘    要:目的:研究江浙蝮蛇蛇毒蛋白诱导K562细胞调亡。方法:通过电镜观察蛇毒蛋白作用后K562细胞的形态变化;MTT检测蛇毒蛋白对细胞增值的影响,同时应用流式细胞仪检测细胞凋亡数及其对细胞周期的影响;采用琼脂糖凝胶电泳观测凋亡片断。结果:蛇毒蛋白作用K562细胞后,能显著抑制细胞增值;LC50为4.96μg/mL,电镜可观察到凋亡形态学改变;电泳呈现典型的阶梯状条带,流式细胞仪检测到凋亡峰。结论:江浙蝮蛇蛇毒蛋白可诱导K562细胞调亡。

关 键 词:蛇毒  K562细胞  江浙蝮蛇  调亡  凋亡  诱导  流式细胞仪检测  毒蛋白  LC50  电镜观察

STUDIES ON THE ANTI-TUMOR ACTIVITY OF A PROTEIN FROM AGKISTRODOM HALYS PALLAS VENOM
Abstract.STUDIES ON THE ANTI-TUMOR ACTIVITY OF A PROTEIN FROM AGKISTRODOM HALYS PALLAS VENOM[J].Natural Product Research and Development,2004,16(5):387-390.
Authors:Abstract
Abstract:Aim: To study the protein purified from Agkistrodom halys Pallas venom might induce apoptosis in K562 cells. Methods: Inhibitions of proliferation was measured using MTT assay. The amount of apoptotic cells was measured by flow cytometry. DNA fragmentation was visualized by DNA agarose gel electrophoresis, and the cellular changes were observed with electron microscope. Results:The protein purified from Agkistrodom halys Pallas venom elicited typical apoptosis morphological changes and DNA fragmentation in a concentration-dependent manner in K562 cells. The protein inhibited K562 cells proliferation in concentration-dependent manner, the LC50 is 4.96μg/ mL. Conclusion:The protein purified from Agkistrodom halys Pallas venom induced apoptosis in K562 cells.
Keywords:Agkistrodom halys Pallas venom  anti-tumor  K562 cells
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