海金沙草多糖的提取及抗氧化活性(英文)

用响应面优化技术研究了提取时间、固液比、提取温度等对海金沙草粗多糖提取的定量影响,获得了提取工艺的最优工艺参数:提取时间为123.3 min,固液比(s/w)为1∶20.9,提取温度为49.9℃,二阶多项式曲线回归模型预测多糖产量为12.466%,多糖提取验证试验结果(提取率)为12.85±0.18%(n=3),比模型预测稍高。多糖经纯化并进行体外抗氧化活性研究(以维生素C为对照品),结果发现,多糖产物对超氧阴离子(O2–.)和羟基氧自由基(.OH)具有较好的清除作用。

Extraction and Antioxidation of Polysaccharides from Lygodium japonicum(Thumb.) Sw

The quantitative effects of extraction time(ETm),the ratio of sample to water(S/W),and extraction temperature(ETp) on polysaccharide extraction rate from Lygodium japonicum(Thumb.) Sw were investigated using response surface methodology(RSM),and the optimum extraction parameters were determined:ETm of 123.3 min,S/W of 1∶20.9(w/v) and ETp of 49.9 ℃.The predicted PS extraction rate reached 12.446%,and the result of verification experiment is 12.85±0.18%(n=3).The antioxidant activities of the purified PS in vitro were evaluated and compared to standard antioxidant as Vitamin C.The results indicated that the purified PS showed strong antioxidant activities against O–·2 and ·OH.