山楝枝叶中两个新的倍半萜

本文报道了对山楝Aphanamixis polystachya枝叶的化学成分进行研究。采用硅胶柱色谱、Sephadex LH-20、RP-C 18、HPLC等色谱分离手段,从其95%乙醇提取物中分离得到9个化合物,通过波谱方法进行鉴定为polystachyadione A(1)、polystachyadione B(2)、labd-13(E)-ene-8α,15-diol(3)、labd-13(E)-ene-8α,15-yl acetate(4)、1 S,4 S,5 S,10 R-4,10-愈创木二醇(5)、齐墩果酸(6)、3,4-二羟基苯甲酸(7)、反式咖啡酸(8)和单棕榈酸甘油酯(9)。其中化合物1和2为两个新化合物,化合物4、7、8和9为首次从该植物中分离得到。化合物1、2对脂多糖诱导的RAW 264.7细胞NO生成没有表现出明显的抑制作用。

Two new sesquiterpene from stems and leaves of Aphanamixis polystachya

To investigate the chemical constituents of the branches and leaves of Aphanamixis polystachya.Nine compounds were isolated from the 95%ethanol extract of A.polystachya by various column chromatography involving silica gel,Sephadex LH-20 gel,RP-C 18 and HPLC.Their structures were identified by chemical properties and spectroscopic analyses,and were elucidated as polystachyadione A(1),polystachyadione B(2),labd-13(E)-ene-8α,15-diol(3),labd-13(E)-ene-8α,15-yl acetate(4),1 S,4 S,5 S,10 R-4,10-guaianediol(5),oleanolic acid(6),3,4-dihydroxybenzoic acid(7),caffeic acid(8)andα-monopalmitin(9),of which compounds 1 and 2 are new.Compounds 4,7,8 and 9 are obtained from this plant for the first time.The activity tests showed that compounds 1 and 2 have no significant inhibition against NO production.