红花降糖活性成分谱效关系研究

红花为菊科植物红花(Carthamus tinctorius L.)的干燥花,具有活血通经,散瘀止痛等功能。为研究其降糖活性成分及谱效关系,本文确定了红花降糖活性部位;建立了HPLC指纹图谱,通过对照品指认其中7个主要成分后,用灰色关联度法和正交偏最小二乘法分析,揭示了这7个共有峰协同发挥蛋白酪氨酸磷酸酯酶1B抑制作用,其中羟基红花黄色素A(HSYA,6号峰)和6-羟基山柰酚-3,6-二-O-葡萄糖基-7-O-葡萄糖苷(3号峰)是贡献最大的成分。含量测定显示10批红花中羟基红花黄色素A及山柰素的含量均在1.67%~1.94%和0.09%~0.12%之间,该研究为红花在糖尿病药物开发领域的应用提供了依据。

Spectrum-effect relationship of antidiabtic extract from the flowers of Carthamus tinctorius L.

The flowers of Carthamus tinctorius L.(Compositae family)have effect on activating blood to promote menstruation,promoting blood circulation to remove blood stasis etc.In order to study the spectrum-effect relationship of antidiabtic extract from the flowers of C.tinctorius,anti-diabetic effect was identified in vitro,HPLC was used to establish fingerprints of extracts of the flowers of C.tinctorius,7 characteristic compounds were identified by chemical reference substances and the PTP1B was used as a marker to perform spectrum-effect relationship analysis.GAR and PLS analysis shown 7 peaks have synergistic effect on PTP1B inhibition.Among them,hydroxysafflor yellow A(HSYA,peak 6)and 6-hydroxykaempferol-3,6-di-D-glucose-7-O-glucoside(peak 3)are the most contributory components.Quantitative analyses shown the content of HSYA and kaempferide in the flowers of C.tinctorius come from different area in Xinjiang were between 1.67%-1.94%and 0.09%-0.12%respectively.All these work provide support for research and development of the flowers of C.tinctorius.