HPLC-DAD法测定血满草中熊果酸和齐墩果酸的含量(英文)

建立了HPLC-DAD法测定血满草中熊果酸和齐墩果酸含量,并进行方法学考察。采用HPLC-DAD进行分析,fusion-RP C18柱(4.6 mm×250 mm,4μm),甲醇-0.2%磷酸水溶液(90∶10)为流动相,检测波长210 nm,体积流量1.0 mL/min。同时采用微波辅助提取、回流提取、索氏提取、冷浸提取、超声提取五种方法对血满草中熊果酸和齐墩果酸含量进行测定并比较不同方法所得结果的差异,还比较了血满草不同部位中熊果酸和齐墩果酸的含量差异。测定结果表明熊果酸进样量在3.6～8.4μg范围内,齐墩果酸进样量在3.2～16μg范围内,呈良好线性关系。血满草中熊果酸和齐墩果酸平均回收率分别为98.3%和101.4%(n=5),相对标准偏差分别为1.13%和0.72%(n=5)。五种方法比较得出索氏提取得熊果酸和齐墩果酸含量最高;血满草花中熊果酸和齐墩果酸含量最高,而根中含量最低。该方法使血满草中熊果酸和齐墩果酸达到基线分离,操作简便,结果稳定可靠。

Determination of Ursolic Acid and Oleanolic Acid in Sambucus adnata Wall by HPLC-DAD

The study aimed to determine the content of ursolic acid and oleanolic acid in Sambucus adnata Wall using HPLC-DAD.By this method,fusion-RP C18(4.6 mm×250 mm,4 μm) was used with methanol-0.2% phosphate in water(90∶10) as the mobile phase and UV detection was at 210 nm.In addition,five different extraction methods including microwave-assisted extraction,reflexing extraction,soxhlet extraction,soaking extraction,and ultrasonic extraction were used for determine the content of ursolic acid and oleanolic acid in different parts of S.adnata Wall and the results were compared.Within the injection volume range between 3.6 and 8.4 μg for ursolic acid and between 3.2 and 16 μg for oleanolic acid,the results had good linearity.The average recovery was 98.3%(RSD=1.13%,n=5) for ursolic acid and 101.4%(n=5)(RSD= 0.72%,n=5)for oleanolic acid.The highest content of ursolic acid and oleanolic acid was obtained by soxhlet extraction methods comparing with other methods.In addition,the flower of S.adnata Wall had the highest content of ursolic acid and oleanolic acid and the root had the lowest amount.Conclusively,the HPLC-DAD is a stable and credible method for determining the content of ursolic acid and oleanolic acid in S.adnata Wall with satisfactory baseline separation for these two kinds of acids.