| 重组人白介素-11蛋白浓度两种测定方法比较 |
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| 引用本文： | 张铁雨,刘忠荣,周正质,吴洽庆.重组人白介素-11蛋白浓度两种测定方法比较[J].天然产物研究与开发,2005,17(6):750-751. |
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| 作者姓名： | 张铁雨 刘忠荣 周正质 吴洽庆 |
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| 作者单位： | 1. 浙江省药品检验所,杭州,310004
2. 成都地奥制药集团有限公司,成都,610041 |
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| 摘 要： | 在基因工程蛋白药物开发研究过程中，选定恰当、准确的蛋白浓度测定方法非常重要。本文比较Lowry法和Bradford法测定重组人白介素-11蛋白浓度结果，认为选用Bradford法是可行的，但中国药典三部蛋白浓度测定方法中没有收录Bradford法，建议在中国药典三部修订时收录该方法。
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| 关 键 词： | 人白介素11浓度测定 Lowry法 Bradford法 |
| 收稿时间： | 2005-07-05 |
| 修稿时间： | 2005-08-15 |
Methods Comparison of Interleukin-11 Concentration Quantification |
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| ZHANG Tie-yu,LIU Zhong-rong,ZHOU Zheng-zhi,WU Qia-qing.Methods Comparison of Interleukin-11 Concentration Quantification[J].Natural Product Research and Development,2005,17(6):750-751. |
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| Authors: | ZHANG Tie-yu LIU Zhong-rong ZHOU Zheng-zhi WU Qia-qing |
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| Institution: | 1. Zhejiang Institute for Drug Control, Hangzhou 310004, China; 2. Chengdu DI-AO Pharmaceutical Group Co. Ltd., Chengdu 610041, China |
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| Abstract: | It is very important to get an optimum method for the quantification of protein during R&D of recombinant protein medicine.The comparison between Lowry and Bradford revealed that Bradford is a practical way for the quantification of IL-11,but the Bradford Dye method was not adopted in the appendixes of Part 3 of Chinese Pharmacopoeia.We suggest that this method for the quantification of protein be adopted in the next revision Part 3 of Chinese Pharmacopoeia. |
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| Keywords: | quantification of recombinant interleukin-11 Lowry method Bradford Dye method |
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