| 响应面法优化混合酶-超声波联合提取栀子黄色素工艺研究 |
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| 引用本文： | 胡居吾,李雄辉,熊伟,季清荣,涂招秀,李向阳.响应面法优化混合酶-超声波联合提取栀子黄色素工艺研究[J].天然产物研究与开发,2011,23(3):551-555. |
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| 作者姓名： | 胡居吾 李雄辉 熊伟 季清荣 涂招秀 李向阳 |
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| 作者单位： | 1. 江西省科学院应用化学研究所
2. 江西省科院天工科技有限责任公司,南昌,330029 |
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| 基金项目： | 农业科技成果转化资金项目 |
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| 摘 要： | 以栀子为原料提取栀子黄色素,将混合酶-超声波技术相结合,对栀子黄色素进行了提取的研究。为了获得提取栀子黄色素最佳工艺条件,采用响应面法组合设计试验方法,建立了酶的用量、提取温度、提取时间之间关系。结果表明,最佳工艺条件为酶的用量4.33%,提取温度61.84℃,提取时间65.06 min,在此最佳提取条件下,栀子黄色素的吸光度为0.914。
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| 关 键 词： | 栀子黄色素 响应面分析 超声提取 |
Optimization of Mixed-Enzymes and Ultrasonic Extraction Technology of Gardenia Yellow by Response Surface Method |
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| HU Ju-wu,LI Xiong-hui,XIONG Wei,JI Qing-rong,TU Zhao-xiu,LI Xiang-yang.Optimization of Mixed-Enzymes and Ultrasonic Extraction Technology of Gardenia Yellow by Response Surface Method[J].Natural Product Research and Development,2011,23(3):551-555. |
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| Authors: | HU Ju-wu LI Xiong-hui XIONG Wei JI Qing-rong TU Zhao-xiu LI Xiang-yang |
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| Institution: | HU Ju-wu1,LI Xiong-hui1,XIONG Wei1,JI Qing-rong2,TU Zhao-xiu1,LI Xiang-yang1 1Institute of Applied Chemistry,Jiangxi Academy of Sciences,2Tian-Gong Technology Co.,Ltd.of Academy of Sciences,Nanchang 330029,China |
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| Abstract: | The extraction of gardenia yellow from Gardenia jasminoides Ellis was studied in this paper and gardenia yellow was extracted by mixed-enzymes and ultrasonic extraction technology,and the mathematical model of correlation among enzyme dosage,extraction temperature,extraction time were established by means of response surface method.The results indicated that the optimum extraction conditions are as follows:enzyme dosage 4.33%,extraction temperature 61.84 ℃,and extraction time 65.06 min.Under these conditions,the absorbance of gardenia yellow is 0.914. |
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| Keywords: | gardenia yellow response surface method ultrasonic extraction |
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