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| 魔芋神经酰胺的提取及其鞘磷脂含量的测定 | |
| 引用本文： | 刘仁萍,杨建奎,颜焱娜,吴民熙,肖鹜轩,吴永尧.魔芋神经酰胺的提取及其鞘磷脂含量的测定[J].天然产物研究与开发,2011,23(3):542-546. |
| 作者姓名： | 刘仁萍 杨建奎 颜焱娜 吴民熙 肖鹜轩 吴永尧 |
| 作者单位： | 1. 湖南农业大学生物科学技术学院 2. 湖南农业大学理学院 3. 湖南省农业科学研究院,长沙,410128 |
| 基金项目： | 湖南省科技计划重大专项 |
| 摘 要： | 采用回流提取法提取魔芋神经酰胺进行初步的探索,重点分析了溶剂浓度、温度、时间、料液比对魔芋神经酰胺提取量的影响,确定了最佳的提取工艺;并且结合高效液相/蒸发光散射检测器的方法对魔芋结合态神经酰胺-鞘磷脂进行了定性定量分析。 |
| 关 键 词： | 魔芋 鞘磷脂 高效液相色谱-蒸发光散射器 |
Extraction of Ceramide from Konjac and Its Sphingomyelin Analysis |
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| LIU Ren-ping,YANG Jian-kui,YAN Yan-na,WU Min-xi,XIAO Wu-xuan,WU Yong-yao.Extraction of Ceramide from Konjac and Its Sphingomyelin Analysis[J].Natural Product Research and Development,2011,23(3):542-546. | |
| Authors: | LIU Ren-ping YANG Jian-kui YAN Yan-na WU Min-xi XIAO Wu-xuan WU Yong-yao |
| Institution: | LIU Ren-ping1,YANG Jian-kui2,YAN Yan-na1,WU Min-xi1,XIAO Wu-xuan3,WU Yong-yao1 1College of Bioscience and Biotechnology,Hunan Agriculture University,2Science of College of Hunan Agriculture University,3Hunan Academy of Agriculture sciences,Changsha 410128,China |
| Abstract: | The extration of ceramide from konjac by the refluxing method was studied.The effects of temperature,time,proportion of solute to solvent and extracting solvent on the extraction efficiencies were analyzed.The optimum extraction conditions were made. Combined with HPLC and evaporative light scattering detector(HPLC/ELSD),The sphingomyelin of konjac were analyzed qualitatively and quantitatively. |
| Keywords: | konjac sphingomyelin HPLC-ELSD |
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