| 酶解鹿茸血ACE抑制活性及其与抗氧化活性关系的研究 |
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| 引用本文： | 任建东,施用晖,马立芹,尹晓平,姜红,乐国伟.酶解鹿茸血ACE抑制活性及其与抗氧化活性关系的研究[J].天然产物研究与开发,2010,22(1). |
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| 作者姓名： | 任建东 施用晖 马立芹 尹晓平 姜红 乐国伟 |
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| 作者单位： | 1. 江南大学食品学院营养与食品功能因子研究所,无锡,214122
2. 食品科学与技术国家重点实验室江南大学;江南大学食品学院营养与食品功能因子研究所,无锡 214122 |
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| 基金项目： | 国家自然科学基金，863项目 |
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| 摘 要： | 实验采用木瓜蛋白酶和Alcalase碱性蛋白酶水解鹿茸血,在不同的时间取样,分别测定血管紧张素转化酶(ACE)抑制率、二苯代苦味肼基自由基(DPPH.)清除率和羟自由基(.OH)清除率。结果表明Alcalase蛋白酶水解产物ACE抑制活性和DPPH.清除活性较强,其水解3h的产物ACE抑制率为90.63%,DPPH.清除率为14.40%;木瓜蛋白酶水解5h的产物.OH清除率最高,为96.7%。用SPSS10.0软件分析结果,发现ACE抑制率与DPPH.清除率具有显著相关性。
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| 关 键 词： | 鹿茸血 ACE抑制活性 羟自由基 DPPH自由基 相关性 |
Studies on ACE Inhibitory Activity and Its Relationship with Antioxidative Activity of Red Deer Antler Blood Hydrolysates |
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| REN Jian-dong,SHI Yong-hui,MA Liqin,YIN Xiao-ping,JIANG Hong,LE Guo-wei.Studies on ACE Inhibitory Activity and Its Relationship with Antioxidative Activity of Red Deer Antler Blood Hydrolysates[J].Natural Product Research and Development,2010,22(1). |
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| Authors: | REN Jian-dong SHI Yong-hui MA Liqin YIN Xiao-ping JIANG Hong LE Guo-wei |
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| Institution: | REN Jian-dong2,SHI Yong-hui1,2,MA Li-qin2,YIN Xiao-ping2,JIANG Hong2,LE Guo-wei1,2 1State Key Laboratory of Food Science , Technology,Jiangnan University,2Institute of Nutrition , Food Safety,School of Food Science , Technology,Wuxi 214122,China |
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| Abstract: | Red Deer antler blood hydrolysates were prepared by enzymatic hydrolysis with alcalase and papain.The in-
hibitory rate of angiotensin Ⅰ-converting enzyme (ACE) and clearance rate of·OH and DPPH·were investigated at
different times of hydrolysis.The highest ACE inhibitory rate and DPPH·clearance rate were obtained with alcalase,
which were 90.63％ and 14.40％ respectively, and the highest·OH clearance rate was obtained with papain, which
was 96.7％.Analyzed by SPSS10.0, the correhtion between ACE inhibitory activity and DPPH·clearance rate was
significant at 0.05 level,while there was on significant relationship between ACE and·OH. |
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| Keywords: | red deer antler blood ACE inhibitory activity OH DPPH relativity |
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