| 马铃薯蛋白酶A抑制剂的纯化与性质 |
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| 引用本文： | 李泉,田亚平.马铃薯蛋白酶A抑制剂的纯化与性质[J].天然产物研究与开发,2008,20(6). |
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| 作者姓名： | 李泉 田亚平 |
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| 作者单位： | 江南大学工业生物技术教育部重点实验室,无锡,214122 |
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| 摘 要： | 采用硫酸铵沉淀、Sephadex G-50和CM柱层析，从马铃薯汁中纯化蛋白酶A（protease A，PrA）抑制剂，并对其部分性质进行研究。蛋白酶A抑制剂为单亚基，相对分子质量为16．71kDa。热稳定性良好，抑制最佳pH范围为5—5．5，最佳反应时间为1．5h，对PrA抑制类型为竞争和非竞争性混合抑制作用。
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| 关 键 词： | 蛋白酶A 抑制剂 分离纯化 |
Purification and Characters of Protease A Inhibitor from Potato |
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| LI Quan,TIAN Ya-ping.Purification and Characters of Protease A Inhibitor from Potato[J].Natural Product Research and Development,2008,20(6). |
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| Authors: | LI Quan TIAN Ya-ping |
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| Institution: | LI Quan,TIAN Ya-ping*The Key Laboratory of Industrial Biotechnology,Ministry of Education,Jiangnan University,Wuxi 214122,China |
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| Abstract: | Protease A inhibitor was purified from potato juice by ammonium sulfate,Sephadex G-50 filtration and CM chromatography.Parts of its characters were analyzed.The results showed that the inhibitor had one subunit,and its relative molecular quality was 16.71 kDa.The inhibitor had a good stability,the best pH limit of inhibition was 5-5.5,the best reaction time was 1.5 h,and the inhibition type was the complex of non-competitive and competitive inhibition. |
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| Keywords: | A |
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