青龙衣中细胞毒活性成分的研究

利用各种化学及色谱技术从青龙衣中分离得到11个化合物,通过理化性质和波谱学手段分别鉴定为2-羟基-1,4-萘醌(2-hydroxy-1,4-naphthoquinone,1)、5-羟基-1,4-萘醌(5-hydroxy-1,4-naphthoquinone,2)、2,5-二羟基-1,4-萘醌(2,5-dihydroxy-1,4-naphthoquinone,3)、3,5-二羟基-1,4-萘醌(2,5-dihydroxy-1,4-naphthoquinone,4)、5,8-二羟基-1,4-萘醌(2,5-dihydroxy-1,4-naphthoquinone,5)、5-甲氧基-1,4-萘醌(5-methoxy-1,4-naphthoquinone,6)、5,7-二羟基色原酮(5,7-dihydroxychromone,7)、异香草酸(isovanillic acid,8)、没食子酸(gallic acid,9)、β-谷甾醇(β-sitosterol,10)和β-胡萝卜苷(β-daucosterol,11)。化合物3、4、6～8为首次从该属植物中分离得到,化合物1为首次从该植物中分离得到。细胞毒活性测试结果表明,化合物3和4对HepG2细胞表现出强的抑制作用,IC50值分别为5.0±0.6μmol/L和7.0±0.5μmol/L;2和5能显著抑制HL-60细胞的增殖,IC50值分别为9.3±1.2μmol/L和2.3±0.2μmol/L。

Chemical Constituents of Pericarpium Juglantis

Eleven compounds were isolated from Pericarpium Juglantis,and their structures were identified as 2-hydroxy-1,4-naphthoquinone（1）,5-hydroxy-1,4-naphthoquinone（2）,2,5-dihydroxy-1,4-naphthoquinone （3）,2,5-dihydroxy-1,4-naphthoquinone（4）,2,5-dihydroxy-1,4-naphthoquinone（5）,5-methoxy-1,4-naphthoquinone （6）,5,7-dihydroxychromone（7）,isovanillic acid（8）,gallic acid（9）,β-sitosterol（10）,and β-daucosterol（11）.by means of their physic-chemical properties and spectroscopic ways.In the cytotoxic assay,3 and 4 exhibited significant inhibitory effects against HepG2 cancer cell lines with IC₅0 value of 5.0±0.6 μmol/L and 7.0±0.5 μmol/L,respectively.And 2 and 5 showed significant inhibitory effects against HL-60 cancer cell lines with IC₅0 value of 9.3 ± 1.2 μmol/L和2.3 ± 0.2 μmol/L,respectively.