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反相高效液相色谱法测定升麻药材中升麻苷H-1的含量
引用本文:姚梅芬,王岳峰,李展,潘瑞乐,赵晓宏,斯建勇.反相高效液相色谱法测定升麻药材中升麻苷H-1的含量[J].天然产物研究与开发,2011,23(4):696-699.
作者姓名:姚梅芬  王岳峰  李展  潘瑞乐  赵晓宏  斯建勇
作者单位:1. 中国医学科学院北京协和医学院药用植物研究所,北京100193,西南交通大学药学院,成都610031
2. 西南交通大学药学院,成都,610031
3. 中国医学科学院北京协和医学院药用植物研究所,北京,100193
基金项目:国家药典委员会项目-2010年版药典增修订
摘    要:建立升麻药材中升麻苷H-1含量测定的反相高效液相色谱分析方法。使用H&E XP ODS-A色谱柱(4.6 mm×250 mm5,μm),流动相为乙腈-水-磷酸(35∶65∶0.4),流速为1 mL/min,柱温为26℃,检测波长203nm。测得升麻苷H-1线性范围为0.95~28.5μg/mL(r=0.9999),平均回收率(n=6)为99.35%(RSD=2.79%),不同来源批次升麻药材含量测定结果表明,升麻药材中升麻苷H-1含量为0.53%~1.09%,综合分析批样品含量测定结果,建议升麻药材中含升麻苷H-1应不低于0.4%。本方法简便、准确、重复性好、专属性强,可作为升麻药材质量控制方法。

关 键 词:升麻  升麻苷H-1  高效液相色谱法  含量测定

Determination of Cimicifugoside H-1 in Rhizoma Cimicifugae by RP-HPLC
YAO Mei-fen,WANG Yue-feng,LI Zhan,PAN Rui-le,ZHAO Xiao-hong,SI Jian-yong.Determination of Cimicifugoside H-1 in Rhizoma Cimicifugae by RP-HPLC[J].Natural Product Research and Development,2011,23(4):696-699.
Authors:YAO Mei-fen  WANG Yue-feng  LI Zhan  PAN Rui-le  ZHAO Xiao-hong  SI Jian-yong
Institution:YAO Mei-fen1,2,WANG Yue-feng2,LI Zhan1,PAN Rui-le1,ZHAO Xiao-hong1,SI Jian-yong1 1Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100193,China,2Southwest Jiaotong University,Chengdu 610031
Abstract:To establish an RP-HPLC method for determination of cimicifugoside H-1 in Rhizoma Cimicifugae.H&E XP ODS-A(4.6×250 mm,5 μm) column was employed with acetonitrile-water-phosphoric acid(35∶ 65∶ 0.4) as mobile phase,the flow rate was 1.0 mL/min.The column temperature was set 26 ℃ and detection wavelength was set at 203 nm.The linear range of cimicifugoside H-1 was 0.95~28.5 μg,the correlation coefficient was 0.9999 and the average recoveries(n=6) was 99.35%.The method is simple,rapid and reliable,which can be regarded as a reasonable method for determining the content of cimicifugoside H-1 in Rhizoma Cimicifugae.The results can be supported as data bases for developing quality specification of Rhizoma Cimicifugae.
Keywords:Rhizoma Cimicifugae  cimicifugoside H-1  HPLC  quantitative determination  
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