| HPLC测定葛花中6″-O-木糖鸢尾苷的含量 |
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| 引用本文： | 姚美村,廖祎婷,袁月梅,高越,汤毅.HPLC测定葛花中6″-O-木糖鸢尾苷的含量[J].天然产物研究与开发,2011,23(5):898-900,938. |
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| 作者姓名： | 姚美村 廖祎婷 袁月梅 高越 汤毅 |
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| 作者单位： | 中山大学药学院药,广州,510006 |
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| 基金项目： | 中山大学2008年实验室开放基金项目″葛花异黄酮类成分的药动/药效学研究″资助 |
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| 摘 要： | 本文建立了葛花中6″-O-木糖鸢尾苷含量测定的HPLC方法。采用GRACE C18(250 mm×4.6 mm,5μm)色谱柱,流动相为乙腈-水梯度洗脱,流速为0.8 mL/min,紫外检测波长为265 nm,柱温为室温。6″-O-木糖鸢尾苷的峰面积(Y)与浓度(X)在10.33～185.99μg/mL范围内线性关系良好,Y=30731X–216635,r=0.9992(n=7);平均回收率为100.2%,RSD<2%(n=9);测得8批葛花药材中6″-O-木糖鸢尾苷含量为11.08～48.23 mg/g。方法学验证结果表明该法准确、可靠,可用于葛花中主要成分6″-O-木糖鸢尾苷的含量测定。
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| 关 键 词： | 葛花 6″-O-木糖鸢尾苷 高效液相色谱法 解酒 不同产地 |
Determination of 6"-O-xylosyl-tectoridin in Flos Puerariae by HPLC |
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| YAO Mei-cun,LIAO Yi-ting,YUAN Yue-mei,GAO Yue,TANG Yi.Determination of 6"-O-xylosyl-tectoridin in Flos Puerariae by HPLC[J].Natural Product Research and Development,2011,23(5):898-900,938. |
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| Authors: | YAO Mei-cun LIAO Yi-ting YUAN Yue-mei GAO Yue TANG Yi |
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| Institution: | YAO Mei-cun,LIAO Yi-ting,YUAN Yue-mei,GAO Yue,TANG Yi School of Pharmaceutical Sciences,Sun Yat-sen University,Guangzhou 510006,China |
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| Abstract: | An HPLC method for determination of 6″-O-xylosyl-tectoridin in Flos Puerariae was developed.The samples were separated on a C18(250 mm × 4.6 mm,5 μm) column with the mobile phase of acetonitrile-water(gradient elution).Flow rate was 0.8 mL/min.The detection wavelength was set at 265 nm.Column temperature was ambient temperature.The linear range of 6″-O-xylosyl-tectoridin was 10.33-185.99 μg/mL,and the linear function was Y = 30731X – 216635(r = 0.9992).The average recovery was 100.2%,and RSD was less than 2%(n = 9).The contents of 6″-Oxylosyl-tectoridin in Flos Puerariae was 11.08-48.23 mg/g.The method was accurate and reliable,which can be used to evaluate the qualities of Flos Puerariae. |
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| Keywords: | Flos Puerariae 6″-O-xylosyl-tectoridin HPLC hangover alleviating different origins of production |
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