| 高速逆流色谱对大黄蒽醌类成分的分离研究 |
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| 引用本文： | 袁萍,周东斌,林顺权,高俊飞,何浏,袁晓.高速逆流色谱对大黄蒽醌类成分的分离研究[J].天然产物研究与开发,2011,23(4):739-741,733. |
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| 作者姓名： | 袁萍 周东斌 林顺权 高俊飞 何浏 袁晓 |
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| 作者单位： | 1. 中国科学院武汉植物园,武汉,430074
2. 牌牌科技有限公司,广州,510540 |
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| 摘 要： | 利用高速逆流色谱对大黄中的5个蒽醌活性成分进行了分离,当两相溶剂系统的组成是石油醚∶乙酸乙酯∶甲醇∶水=8∶2∶8∶1时,分离出大黄素;当两相溶剂比为3∶4∶3∶2时,分离出大黄酸和芦荟大黄素;当溶剂比为12∶2∶12∶1时,分离出大黄酚和大黄素甲醚;经高压液相色谱检测大黄素、大黄酸和芦荟大黄素、大黄酚和大黄素甲醚的含量分别为98.81%9、9.15%、98.51%9、8.89%和98.16%。
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| 关 键 词： | 高速逆流色谱 芦荟大黄素 大黄酸 大黄素 大黄酚 大黄素甲醚 分离 |
Separation of Anthraquinone from Rhubarb by High Speed Countercurrent Chromatography |
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| YUAN Ping,ZHOU Dong-bin,LIN Shun-quan,GAO Jun-fei,HE Liu,YUAN Xiao.Separation of Anthraquinone from Rhubarb by High Speed Countercurrent Chromatography[J].Natural Product Research and Development,2011,23(4):739-741,733. |
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| Authors: | YUAN Ping ZHOU Dong-bin LIN Shun-quan GAO Jun-fei HE Liu YUAN Xiao |
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| Institution: | YUAN Ping1,ZHOU Dong-bin2,LIN Shun-quan1,GAO Jun-fei1,HE Liu1,YUAN Xiao1 1Wuhan Botanical Garden,Chinese Academy of Sciences,Wuhan 430074,China,2PI & PI Technologies Inc.,Guangzhou 510540 |
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| Abstract: | Five anthraquinones from Rhubarb were separated by high speed countercurrent chromatography.When two-phase solvent system composition was petroleum ether:ethyl acetate:methanol:water(8∶ 2∶ 8∶ 1),emodin was obtained.Rhein and aloe emodin were separated when the proportion of solvents was 3∶ 4∶ 3∶ 2,while chrysophanol and rheochrysidin were separated at the proportion of solvents 12∶ 2∶ 12∶ 1.The purities of emodin,rhein,aloe emodin,chrysophanol and rheochrysidin are 98.81%,99.15%,98.51%,98.89% and 98.16% respectively by HPLC. |
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| Keywords: | high speed countercurrent chromatography aloe emodin rhein emodin chrysophanol rheochrysidin separation |
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