提取工艺和植物生长调节剂对罗布麻愈伤组织中黄酮含量的影响

以罗布麻愈伤组织粉末为材,在单因素实验的基础上,利用响应曲面法对罗布麻愈伤组织中黄酮的提取工艺进行优化。响应曲面分析结果表明,提取试剂和提取温度对提取的黄酮含量存在显著影响。通过响应曲面分析得到罗布麻愈伤组织中黄酮提取的最佳条件为:提取试剂为70%甲醇,物料比1∶40,提取时间为4 h,提取温度为70℃。培养并比较了30种不同植物生长调节剂浓度与配比诱导100 d生长的愈伤组织中黄酮的含量,结果得出MB+KT(1.0 mg/L)+NAA(0.2 mg/L)上培养约100 d的愈伤组织中黄酮含量最高,为73.90mg/g。测定愈伤组织培养30 d内黄酮的积累动态,探明从培养的愈伤组织提取黄酮的最佳时段。通过优化提取工艺和筛选最佳植物生长调节剂浓度与配比,运用组织培养技术提高了罗布麻愈伤组织中黄酮含量。

Effect of Extraction Process and Plant Growth Regulators on Flavonoids Content in Callus of Apocynum venetum

Optimization of flavonoids extraction process carried out in callus of Apocynum venetum through the response surface method after single factor was studied.The method indicated that there were significant effects to flavonoids content in extracting reagent and temperature.The optimal parameter for extracting flavonoids were conformed by analysis of response surface method as follows:the extracting reagent is 70% methanol,the ratio of solid to liquid is 1∶40,the extracting time was 4 h, the extracting temperature is 70 ℃.Flavonoids were measured respectively from the callus induced for 100 d on 30 kinds of different concentration and combination of plant growth regulators.The results showed that the best culture medium was MB+KT(1.0 mg/L)+NAA(0.2 mg/L),flavonoids contain in callus on the medium for 100 d reached 73.90 mg/g.accumulative dynamic state of flavonoids in callus cultured for 30 d were measured continuously,the optimal time of extracting flavonoids was determined.Flavonoids content in callus of A.venetum were increased with tissue culture technology through optimizing extraction process and screening concentration and combination of plant growth regulators.