RP-HPLC法同时测定藏药珠芽蓼中牡荆素、槲皮苷和槲皮素的含量(英文)

采用RP-HPLC法建立了同时测定珠芽蓼中牡荆素、槲皮苷、槲皮素三种黄酮的方法,色谱柱为kromasilC18(250 mm×4.6 mm,5μm),以甲醇-水(含0.25%磷酸)为流动相梯度洗脱,流速1 mL/min,检测波长360 nm。结果表明,牡荆素、槲皮苷和槲皮素质量浓度在8～40、5～50μg/mL和5.33～52μg/mL范围内与色谱峰面积均呈良好的线形关系,平均回收率分别为100.3%、98.9%和100.4%,日内稳定性偏差分别为0.78%,0.51%和0.38%,日间稳定性偏差为2.6%,1.9%和2.1%。该方法简单、准确,可为珠芽蓼的质量控制提供科学依据。

Simultaneous Quantitative Determination of Viterxin, Quercetin and Quercitrin in Polygonum viviparum in Tibet Plateau by RP-HPLC

To develop a 8ensitive and specific reversed-phase high perfonnance liquid chromatography ( RP-HPLC) method for simultaneous detennination of three flavoniods in Polygonum viviparum,the HPLC separation was performed on a Kromasil C18 column (250 mm ×4.6 mm,5 μm) and detected with DAD at 360 nm.The gradient elution was carried out with methano-H2O (containing 0.25％ phosphoric acid) as the mobile phase at a flow rate of 1 mL/min.The results indicated that the calibration cunre8 for viterxin,quercetin and quercitrin were linear over the ranges of 8-40 μg/mL,5-50 μg/mL and 5.33 -52 μg/mL,their assay recoveries were 100.3％,98.9％and 100.4％,their intraday repeatability were 0.78％,0.51％ and 0.38％,and interday repeatability were 2.6％,1.9％ and 2.1％,respectively.This method was simple,accurate and sensitive,so it could be used for the quality control of P.viviparum.<英文关键词>=Key words:Polygonum viviparum ; viterxin ; quercetin ; quercitrin ; RP-HPLC