两面针提取物(S-O)对小鼠镇痛、抗炎和止血作用的研究

本研究对两面针根的提取物S-O进行了镇痛、止血和抗炎药理实验，每种作用选用两种实验方法来评价。镇痛作用采用热板法和扭体法。热板法实验显示，S-O在150mg/kg剂量时，小鼠痛阈值明显提高（P〈0．01）；扭体法实验显示，S-O在150mg/kg剂量为时，对冰醋酸致痛的小鼠扭体反应次数减少了70．96％（P〈0．01）。抗炎实验采用二甲苯致小鼠耳廓肿胀法及腹腔染料渗出法。二甲苯致炎剂实验表明，S-O在150mg/kg剂量时，对二甲苯所致小鼠耳廓肿胀有明显抑制作用，抑制率为63．45％（P〈0．01）；冰醋酸所致的腹腔毛细血管通透性实验中，S-O在150mg/kg和75mg/kg两个剂量组时，对小鼠的抗炎效果分别为52．94％（P〈0．01）和52．00％（P〈0．01）。止血实验采用毛细玻璃管法和载玻片法。毛细玻璃管实验表明S-O在150mg/kg和75mg/kg两个剂量时，凝血时间明显缩短（P〈0．01）；载玻片实验表明S-O在150mg/kg剂量时，凝血时间明显缩短（P〈0．01）。总之，两面针中提取物S-O对小鼠具有显著的镇痛、止血和抗炎作用。

The Effects of the Extract(S-O) from Zanthoxylum nitidum on Analgesia,Anti-inflammation and Hemostasia in Mice

The roots of Zanthoxylum nitidum(Rox. )DC have been commonly used for rheumatism arthritis,strain gall,neuralgia, toothache, stomach angina, and ulcer etc, in traditional Chinese medicine. This study was performed to assess the efficacies of the extract of the roots of Zanthoxylum nitidum(S-0) on analgesia, anti-inflammation and hemostasia in mice. Each efficacy was assessed by two methods. Analgesia was assessed by the methods of hot-plate and writhing test. In hot plate test, 150 mg/kg S-O has significantly increased the pain threshold of mice( P ＜ 0.01 ). In the writhing test, 150 mg/kg S-O, the number of writhes were reduced more than 70.96% (P ＜ 0.01 ). Anti-inflammation was assessed by the methods of xylene induced ear edema and vascular permeability augmentation by acetic acid test. In the xylene induced ear edema, the inhibition of 150 mg/kg S-O was 63.45 ( P ＜ 0.05). In the vascular permeability augmentation by acetic acid test, the inhibition of anti-inflammation of 150 mg/kg,75 mg/kg S-O were 52.94% ( P ＜0.05) ,52.00%( P ＜0.05),respectively,it has significant anti-inflammation.Hemostasia was assessed by the methods of glass tube and glass plate.In glass tube test,both doses of S-O (150 mg/kg, 75 mg/kg) can significantly prolong the blood solidification time ( P ＜ 0.01 ). In the glass plate test, 150 mg/kg S-O can also significantly prolong the blood solidification time( P ＜ 0.01). In conclusion, the S-O of Zanthoxylum nitidum has significant efficacies onanalgesia, anti-inflammation and hemostasia in mice.