| 银杏植物各部位及银杏组织培养细胞中银杏内酯B和白果内酯含量的初步研究 |
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| 引用本文： | 孟超,徐弘,程克棣.银杏植物各部位及银杏组织培养细胞中银杏内酯B和白果内酯含量的初步研究[J].天然产物研究与开发,2005,17(5):603-605. |
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| 作者姓名： | 孟超 徐弘 程克棣 |
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| 作者单位： | 中国医学科学院,中国协和医科大学药物研究所,卫生部天然药物生物合成重点实验室,北京100050 |
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| 摘 要： | 用高压液相色谱法对五年生扦插银杏各部位及银杏组织培养细胞中银杏内酯B和白果内酯的含量进行了测定.结果表明银杏内酯B和白果内酯在银杏植物各部位的含量差异很大.银杏内酯B在银杏叶中含量最高,白果内酯在银杏侧根中含量最高.在6,7-v培养基下银杏组织培养细胞中同时测出银杏内酯B和白果内酯,提示用植物组织培养方法有可能同时产生银杏内酯B和白果内酯.
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| 关 键 词： | 银杏 HPLC 银杏内酯B 白果内酯 组织培养 |
| 收稿时间： | 2004-07-26 |
| 修稿时间： | 2004-10-28 |
Preliminary Study on the Ginkgolide B and Bilobalide Contents in Different Tissues and Callus Cultures of Ginkgo biloba |
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| MENG Chao,XU Hong, CHENG Ke-di.Preliminary Study on the Ginkgolide B and Bilobalide Contents in Different Tissues and Callus Cultures of Ginkgo biloba[J].Natural Product Research and Development,2005,17(5):603-605. |
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| Authors: | MENG Chao XU Hong CHENG Ke-di |
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| Institution: | Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union of Medical College, Beijing 100050, China |
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| Abstract: | HPLC method has been performed to analyze the contents of ginkgolide B and bilobalide in different tissues of fiveyear plants and tissue cultures of Ginkgo biloba. The results showed that the contents of ginkgolide B and bilobalide varied in different parts of the plant. The highest amounts of ginkgolide B and bilobalide were detected in leaves and side roots respectively. Among 3 different cell lines ginkgolide B and bilobalide could only be detected in cell line YX-8 in the medium of 6,7- V. The results suggested that it might be a possible way to produce useful metabolites by cell cultures of Ginkgo biloba. |
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| Keywords: | Ginkgo biloba ginkgolide B bilobalide cell culture HPLC |
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