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| 壳聚糖中胺基对其抑菌性能的影响及与DNA的作用 | |
| 引用本文： | 李小芳,冯小强,伏国庆,杨声,苏中兴.壳聚糖中胺基对其抑菌性能的影响及与DNA的作用[J].天然产物研究与开发,2010,22(3). |
| 作者姓名： | 李小芳 冯小强 伏国庆 杨声 苏中兴 |
| 作者单位： | 1. 天水师范学院,生命科学与化学学院 2. 天水市中医医院化验科,天水,741001 3. 兰州大学化学化工学院,兰州,730000 |
| 基金项目： | 甘肃天水师范学院物理无机化学重点学科基金 |
| 摘 要： | 采用抑菌圈法研究了壳聚糖对大肠杆菌(E.coli)和金黄色葡萄球菌(St.aureus)的抑菌活性。利用壳聚糖的席夫碱反应,对壳聚糖的胺基进行保护后,研究了壳聚糖中胺基对其抑菌性能的影响。同时,运用紫外吸收光谱和电化学的方法,研究了壳聚糖与DNA的相互作用,提出了壳聚糖对E.coli和St.aureus的抑菌机理。研究结果表明,壳聚糖对E.coli和St.aureus具有很好的抑制作用,且抑菌活性与其胺基有关;壳聚糖能与细胞内带负电的核酸结合,使细胞正常DNA复制生理功能受到影响,抑制细菌的繁殖,从而达到抑菌的目的。 |
| 关 键 词： | 壳聚糖 抑菌活性 希夫碱 胺基 |
Interaction of Chitosan with DNA and Effect of Amino Group on Antibacterial Activity |
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| LI Xiao-fang,FENG Xiao-qiang,FU Guo-qing,YANG sheng,SU Zhong-xing.Interaction of Chitosan with DNA and Effect of Amino Group on Antibacterial Activity[J].Natural Product Research and Development,2010,22(3). | |
| Authors: | LI Xiao-fang FENG Xiao-qiang FU Guo-qing YANG sheng SU Zhong-xing |
| Institution: | LI Xiao-fang1,FENG Xiao-qiang1,FU Guo-qing2,YANG sheng1,SU Zhong-xing31College of Life Science and Chemistry,Tianshui Normal University,2Traditional Medical hospital of Tianshui,Tianshui 741001,China,3Chemistry and Chemical Engineering College,Lanzhou University,Lanzhou 730000 |
| Abstract: | The antibacterial activity of chitosan against Escherichia coli and Staphylococcus aureus were investigated by measuring inhibition zone.Aimed to explain the effect of amino group on antibacterial activity of chitosan,four different kinds of chitosan Schiff's bases were synthesized.UV absorption spectroscopy and electrochemical analysis were used to illuminate the interaction of chitosan with DNA.The results showed that the antibacterial ability of chitosan was related to the amino group.Chitosan can bind w... |
| Keywords: | DNA |
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