梨花中熊果苷的高效液相色谱分析

采用高效液相色谱法对不同时期、不同品种的梨花中熊果苷的含量进行了分析,色谱柱为Hypersil BDSC18(4.6 mm×250 mm,5μm),流动相为甲醇∶水(6∶94),加入甲酸0.05%,检测波长280 nm。结果表明:熊果苷在0.01～5.00μg范围内线性关系良好(r=0.9999),平均加标回收率为97.7%。不同生长期的鸭梨梨花中熊果苷的含量一般在10 mg/g以上,尤以花芽萌动期时含量最高,达到35.7 mg/g(鲜重计)。不同品种的9份梨花样品中,熊果苷含量在3.5～10.5 mg/g之间。

Quantification of Arbutin from Pear Blossoms by HPLC

The contents of arbutin in pear blossoms of different periods and different varieties were detected using the HPLC method with Hypersil BDS C18 column(4.6 mm × 250 mm,5 μm),the mobile phase methanol ∶ water(6∶ 94,with 0.05% formic acid),and the detection wavelength 280 nm.The results showed that the calibration curve was linear in the range of 0.01~5.00 μg(r = 0.9999) and the average recovery was 97.7%.The content of arbutin in Yali pear blossom during the florescence was above 10 mg/g(fresh weight) in most cases,especially in blossom bud germination period,reaching a maximum of 35.7 mg/g.In the nine pear blossom samples from different varieties,the contents of arbutin were range between 3.5 mg/g to 10.5 mg/g.