高山红景天细胞悬浮培养中红景天甙生物合成代谢的调控 Ⅰ:前体的影响

探索了高山红景天(Rhodiola sachalinensis A.Bor)细胞培养中红景天甙生物合成的途径,认为甙元酪醇是经由莽草酸途径生成的。在此基础上研究了酪醇、L-酪氨酸与L-苯丙氨酸三种前体加入对红景天甙生物合成的调控作用。结果表明,酪醇、酪氨酸等前体易被多酚氧化酶氧化成褐色,用与前体浓度为1:1的V。来防止褐化效果显著;浓度为0.5mmol/L的酪醇,酪氨酸及苯丙氨酸在细胞培养15d时添加,使红景天甙含量由0.336％分别提高到1.43％、1.11％、0.85％。

REGULATION OF METABOLISM FOR IMPROVED SALIDROSIDE PRODUCTION IN CELL SUSPENSION CULTURE OF RHODIOLA SACHALINENSIS A. BOR I:THE EFFECT OF PRECURSOR

The possible pathway for salidroside biosynthesis in cell suspension culture of Rhodiola sacha-linensis A. Bor was explored. The aglycon tyrosol was suggested to be biosynthesized through the way of shikimic acid cycle. Three precursors for salidroside synthesis, tyrosol, tyrosine and phenylalanine were examined for their effects on cell growth and salidroside accumulation. It was found that tyrosol and tyrosine tended to be oxidized by polyphenol oxidase when added into the culture,as a result the culture broth turned brown. The ascorbic acid(Vc)with concentration equivalent to that of the two precursors added proved to effectively inhibit the activity of polypenol oxidase. In the case of 0. 5mmol/L each of tyrosol,tyrosine and phenylalanine added at day 15,the salidroside content of cultured cells was improved from 0. 336% to 1. 43% ,1. 11% and 0. 85% respectively.