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产黄芩苷内生真菌的筛选与鉴定
引用本文:魏希颖,张黎光,姚玉秀,尚菲,马彩霞,刘清梅.产黄芩苷内生真菌的筛选与鉴定[J].天然产物研究与开发,2012,24(6):819-823.
作者姓名:魏希颖  张黎光  姚玉秀  尚菲  马彩霞  刘清梅
作者单位:1. 陕西师范大学生命科学学院,西安,710062
2. 陕西师范大学生命科学学院,西安710062;陕西省微生物研究所,西安710043
基金项目:陕西师范大学2010重点教学改革研究项目资助课题,中央高校基本科研业务费资助项目
摘    要:从药用植物黄芩根、茎、叶和花中分离得到17株内生真菌,其发酵液对10种指示菌进行抑菌活性测定,并通过薄层色谱(TLC)和高效液相色谱(HPLC)对所分离到的内生真菌代谢产物进行分析。结果显示,所分离到的内生真菌中12株至少对一种指示菌有抑菌活性,其中3株(G2、J4和J5)具有较广的抑菌作用。从黄芩茎和花中分离得到的2株内生真菌J1、H3可以在人工培养基中产生黄芩活性成分——黄芩苷,结合菌落形态特征、显微观察及ITS序列分析,初步鉴定这2株菌株均属于青霉菌属。

关 键 词:黄芩  植物内生真菌  抗菌  黄芩苷

Selection and Identification of Baicalin-Producing Endophytic Fungi Isolated from Scutellaria baicalensis Georgi
WEI Xi-ying , ZHANG Li-guang , YAO Yu-xiu , Shang Fei , MA Cai-xia , LIU Qing-mei.Selection and Identification of Baicalin-Producing Endophytic Fungi Isolated from Scutellaria baicalensis Georgi[J].Natural Product Research and Development,2012,24(6):819-823.
Authors:WEI Xi-ying  ZHANG Li-guang  YAO Yu-xiu  Shang Fei  MA Cai-xia  LIU Qing-mei
Institution:1College of Life Science,Shaanxi Normal University,Xi’an 710062,China; 2Shaanxi Province Institute of Microbiology,Xi’an 710043,China
Abstract:17 endophytic fungi have been isolated from roots,stems,leaves and flowers of the medicinal plant Scutellaria baicalensis Georgi.The antimicrobial activity of the fungal fermentation broth was detected against 10 kinds of indicator bacteria.At the same time,analysis of the metabolites of these endophytic fungi was done by thin layer chromatography(TLC) and high-performance liquid chromatography(HPLC).The results of the antimicrobial test showed that the fermentation broths of the 12 strains of endophytic fungi displayed growth inhibition on at least one test bacterium,some of them(G2,J4,J5) showed a broader antimicrobial spectrum.TLC and HPLC analysis showed that 2 strains of fungi from the stem and flower of Scutellaria baicalensis Georgi produced bioactive component baicalin in the artificial culture medium.Colony morphology of the fungal culture,microscopic observation and ITS sequence analysis together indicated that strains J1 and H3 were classified to Penicillium sp.
Keywords:Scutellaria baicalensis Georgi  endophytic fungi  antimicrobial  baicalin
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