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响应面法优化树脂吸附红枣黄酮工艺研究
引用本文:李治龙,刘新华,刘文杰,冯婷.响应面法优化树脂吸附红枣黄酮工艺研究[J].天然产物研究与开发,2012,24(6):808-813.
作者姓名:李治龙  刘新华  刘文杰  冯婷
作者单位:1. 新疆塔里木大学生命科学学院;塔里木盆地生物资源保护利用兵团重点实验室,阿拉尔843300
2. 塔里木盆地生物资源保护利用兵团重点实验室,阿拉尔,843300
3. 新疆塔里木大学生命科学学院
摘    要:以红枣为原料提取红枣黄酮,通过吸附树脂的筛选,探讨树脂吸附的溶液浓度、pH值、吸附时间、吸附液体积对树脂吸附条件的影响。利用Box-Behnken中心组合设计原理,应用响应曲面分析方法,确定了大孔吸附树脂对红枣黄酮最佳吸附条件为:最佳吸附树脂为XDA-8,吸附时间62 min,吸附液浓度为4.3 mg/L,pH值为7.32,吸附液体积为10 mL,最大吸附效率达到85.64%,优化后得实际吸附率为85.51%。

关 键 词:树脂  红枣  响应面

Optimization of Resins Adsorption Technology for Total Flavonoid from Jujube by Response Surface Methodology
LI Zhi-long , LIU Xin-hua , LIU Wen-jie , FENG ting.Optimization of Resins Adsorption Technology for Total Flavonoid from Jujube by Response Surface Methodology[J].Natural Product Research and Development,2012,24(6):808-813.
Authors:LI Zhi-long  LIU Xin-hua  LIU Wen-jie  FENG ting
Institution:1College of Life Science,Tarim University;2Key Laboratory of Biological Resource Protection and Utilization of Tarim Basin,Xinjiang Production & Construction Group,Alar 843300,China
Abstract:Adsorption and desorption characteristic of different resins for total flavonoid from Jujube was investigated.The experiment was conducted to study the effects of resin adsorption conditions on adsorption rate of flavonoid,and the adsorption time,pH,volume and concentration were developed.By the Box-Behnken centre united experiment design and response surface methodology,the optimum adsorption conditions were developed using resin XDA-8.The adsorption time was 62 min,volume value 10 mL,pH value 7.32 and the concentration was 4 mg/L.Under the optimum condition,the sample adsorption rate was 85.64%,and the actual rate of adsorption was 85.51%.
Keywords:resin  Jujube  response surface methodology
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