枳实中辛弗林和橙皮苷的联合提取工艺研究

以辛弗林和橙皮苷的收率及纯度为指标,研究了含辛弗林的枳实提取物及橙皮苷的综合制备工艺.先采用酸液渗漉法提取枳实中的辛弗林,再采用碱提酸沉法提取原料中的橙皮苷,考察盐酸浓度及用量、流速等因素的影响.辛弗林的最佳提取工艺为:药材加5倍水浸泡过夜,渗漉盐酸液的浓度为0.02 mol/mL,渗漉料液比为1:4,以5 mL/min的流速渗漉;碱提酸沉法提取橙皮苷的优化工艺为:于提取辛弗林之后的药渣中加入65%乙醇,于60℃搅拌条件下加碱调节pH 13,过滤后的滤液以稀盐酸调节pH 5,过滤后再用碱回调pH7,放置过夜.橙皮苷的收率为11.3%,HPLC测定橙皮苷的纯度为86.7%.

Studies on the Integrated Extraction Technology of Synephrine and Hesperidin from Citrus aurantium

The purpme of this paper is to establish one integrated extraction technology of synephrine and hesperidin from Citrus aurantium based on the yield and purity.Extraction protocols such as solvent volume,extraction time,concentration and volume of acid solution,and flow rate of percolation were optimized.The final optimized extraction technology followed as:the materials were crashed to coarse powder,added 5 times water to extrct 24 h and filter,used 4 fold 0.02 mol/mL hydrochloric acid solution as percolate liquor,percolate speed was 5 mL/min,dissolved the filter cakes with 65% alcohol solution,adjusted to pH 13,fihered and adjusted to pH5 with hydrochloric acid solution,modulated pH value of filter solution to 7 and leaved for 12 h.The yield and the purity of hesperidin were 11.3% and 86.7% (HPLC analysis)respectively.