| 甘草酸对猪呼吸道冠状病毒的抗病毒作用 |
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| 引用本文： | 曹敏杰,吴国平,翁凌,苏文金.甘草酸对猪呼吸道冠状病毒的抗病毒作用[J].天然产物研究与开发,2007,19(2):221-224. |
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| 作者姓名： | 曹敏杰 吴国平 翁凌 苏文金 |
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| 作者单位： | 1. 集美大学生物工程学院,厦门,361021
2. 集美大学生物工程学院,厦门,361021;厦门大学生命科学院,厦门,361005 |
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| 基金项目： | This project was supported by a grant from the Bureauof Science and Technology of Fujian Province (2003N083)，agrant from the Bureau of Science and Technology of Xiamen City(3502Z20031054) |
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| 摘 要： | 利用ST细胞研究了甘草酸对猪呼吸道冠状病毒(PRCV)体外复制过程中的抑制作用。通过RT-PCR和Western blot检测发现甘草酸能有效抑制PRCV的体外复制而对ST细胞不引起明显细胞毒作用。抑制效果与甘草酸的剂量成正比关系。PRCV病毒核衣壳蛋白的表达无论在mRNA水平还是在蛋白质水平均随着甘草酸添加量的增加而降低,提示甘草酸是一种有效的抗PRCV药物。
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| 关 键 词： | 甘草酸 核衣壳蛋白 猪呼吸道冠状病毒 |
| 文章编号： | 1001-6880（2007）02-0221-04 |
| 收稿时间： | 2005-10-09 |
| 修稿时间： | 2006-11-29 |
Antiviral Effect of Glycyrrhizin on Porcine Respiratory Coronavirus (PRCV) |
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| CAO Min-jie,WU Guo-ping,WENG Ling,SU Wen-jin.Antiviral Effect of Glycyrrhizin on Porcine Respiratory Coronavirus (PRCV)[J].Natural Product Research and Development,2007,19(2):221-224. |
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| Authors: | CAO Min-jie WU Guo-ping WENG Ling SU Wen-jin |
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| Institution: | 1. School of Biotechnology, Jimei University,Xiamen ,361021, China; 2.School of Life Science ,Xiamen University ,Xiamen ,361005, China |
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| Abstract: | The inhibitory effect of glycyrrhizin (GL) on porcine respiratory coronavirus (PRCV) replication was investigated in vitro in ST cells using RT-PCR and Western blot techniques.GL effectively suppressed PRCV replication without causing apparent cell cytotoxicity in ST cells.The inhibitory effect of GL on PRCV was in a dose-dependent manner.The expression levels of both mRNA and protein of the PRCV nucleocapsid protein in ST cells were impeded by the increasing addition of GL,suggesting it is a potential effective antiviral component against PRCV in vitro. |
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| Keywords: | glycyrrhizin nucleocapsid protein porcine respiratory coronavirus (PRCV) |
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