| β-淀粉样前体蛋白胞内端与JKTBP2蛋白的相互作用 |
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| 引用本文： | 王蔚蔚,李志东,刘润中,洪水根,许华曦,陈晔光.β-淀粉样前体蛋白胞内端与JKTBP2蛋白的相互作用[J].实验生物学报,2005,38(2):164-170. |
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| 作者姓名： | 王蔚蔚 李志东 刘润中 洪水根 许华曦 陈晔光 |
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| 作者单位： | [1]厦门大学生命科学学院，分子细胞神经生物学实验室，厦门361005 [2]清华大学生物科学与技术系，生物膜与膜生物工程国家重点实验室，北京100084 |
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| 摘 要： | β-淀粉样前体蛋白APP(β-amyloid precursor protein)与阿尔茨海默氏症密切相关，它经分泌酶1切割后生成的胞内端AID(APP intracellular domain)能够诱导细胞凋亡。为了研究AID在阿尔茨海默氏症病理过程中的作用，我们以AID为诱饵蛋白用酵母双杂交系统筛选与之有相互作用的蛋白。我们发现人不均一核蛋白D类似蛋白JKTBP2的90-204位肽段可以结合AID。利用293T细胞表达蛋白后进行免疫共沉淀，结果证实二者间存在相互作用。这些结果指出JKTBP2可能在阿尔茨海默氏症形成中有重要作用。
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| 关 键 词： | 淀粉样前体蛋白 相互作用 酵母双杂交系统 免疫共沉淀 AID 细胞凋亡 病理过程 细胞表达 APP 核蛋白 不均一 分泌 酶 |
The interaction of the intracellular domain of beta-amyloid precursor protein with JKTBP2] |
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| Wei Wei Wang,Zhi Dong Li,Run Zhong Liu,Sui Gen Hong,Xua Xi Xu,Ye Guang Chen.The interaction of the intracellular domain of beta-amyloid precursor protein with JKTBP2][J].Acta Biologiae Experimentalis Sinica,2005,38(2):164-170. |
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| Authors: | Wei Wei Wang Zhi Dong Li Run Zhong Liu Sui Gen Hong Xua Xi Xu Ye Guang Chen |
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| Institution: | Laboratory of Molecular Cell Neurological Sciences, School of Life Sciences, Xiamen University, Xiamen 361005. |
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| Abstract: | APP (beta-amyloid precursor protein) plays an important role in the formation of Alzheimer's Disease (AD), and its proteolytic product, the intracellular domain AID is believed to be involved in this process by promoting cell apoptosis. To further understand the function of AID in the pathology of AD, we utilized AID as a bait to identify AID interacting proteins in a yeast two-hybrid system. One of the positive clones encodes the fragment corresponding to amino acids 90-204 of heterogeneous nuclear ribonucleoprotein D-like JKTBP2. The interaction between JKTBP2(90)-204 and AID was further confirmed by co-immunoprecipitation in the mammalian 293T cells. These results indicate that JKTBP2 may have an important function in AD formation. |
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