| 小麦与叶锈菌互作过程中细胞程序性死亡的生化证据（简报） |
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| 引用本文： | 白志英,王冬梅,侯春燕,韩胜芳.小麦与叶锈菌互作过程中细胞程序性死亡的生化证据（简报）[J].实验生物学报,2004,37(4):329-332. |
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| 作者姓名： | 白志英 王冬梅 侯春燕 韩胜芳 |
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| 作者单位： | [1]河北农业大学园林与旅游学院，保定071001 [2]河北农业大学生命科学院，保定071001 |
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| 摘 要： | 细胞程序性死亡(Programmed Cell Death，PCD)是一种受基因调控的、主动的、连续的程序化反应。一般发生程序性死亡的细胞具有较为典型的形态及生化特征：细胞收缩、核染色质浓缩、趋边化；DNA在一定位点被核酸内切酶降解成以180bp为基数的片段，在琼脂糖凝胶电泳中呈现“梯”状条带
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| 关 键 词： | 小麦 叶锈菌 细胞 程序性死亡 DNA 基因 |
Histological observation on programmed cell death in wheat-leaf rust fungus intreaction] |
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| Zhi Ying Bai,Dong Mei Wang,Chun Yan Hou,Sheng Fang Han.Histological observation on programmed cell death in wheat-leaf rust fungus intreaction][J].Acta Biologiae Experimentalis Sinica,2004,37(4):329-332. |
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| Authors: | Zhi Ying Bai Dong Mei Wang Chun Yan Hou Sheng Fang Han |
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| Institution: | College of Landscape Architecture and Tourism, Agricultural University of Hebei, Baoding 071001. |
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| Abstract: | The biochemical character of PCD in different interaction between wheat and Puccinia recondita f. sp tritici was observed using DNA agarose gel electrophoresis and end labeling in situ (Terminal Deoxynucleotidyl Transferase-mediated dUTP Nick End TUNEL). The DNA ladder, which is the typical character of programmed cell death(PCD), was observed in incompatible combination (cv.lorvin10 x race 162) and the positive reaction in wheat leaves of incompatible combination since 12h after inoculation also appeared. The results, coupled with the previous study, indicated that HR was a typical form of PCD in incompatible combination between wheat and wheat leaf fungus. |
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