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植物花粉管中类整联蛋白的免疫荧光定位研究
引用本文:孙颖,穆睿玲.植物花粉管中类整联蛋白的免疫荧光定位研究[J].实验生物学报,1999,32(2):163-168.
作者姓名:孙颖  穆睿玲
摘    要:

关 键 词:整联蛋白  花粉管  细胞外基质  免疫荧光  定位

Immunofluorescence studies on the localization of integrin-like protein in plant pollen tubes]
Y Sun,R L Mu,M P Hao,D Y Sun,Z H Zhang.Immunofluorescence studies on the localization of integrin-like protein in plant pollen tubes][J].Acta Biologiae Experimentalis Sinica,1999,32(2):163-168.
Authors:Y Sun  R L Mu  M P Hao  D Y Sun  Z H Zhang
Institution:Department of Biology, Hebei Normal University, Shijiazhuang 050016.
Abstract:The Strong fluorescence signals were obtained in pollen tube of Lilium davidii Duch with labeled anti-VnR integrin serum, and anti-beta 3, alpha v integrin subunit cytoplasmic domain serum separately. The highest density of immunolabel was in the tip of pollen tube. There was little or no immunolabel in control experiment using non-immune serum, second antibody alone and anti-FnR, LnR integrin serum separately. In pollen of Prunus persica f. rubro-piena Schneid, fluoresence signals were also obtained in tube using labeled anti-beta 1, beta 3 integrin subunit cytoplasmic domain serum separately and in apertures using anti-beta 1 serum. Preliminary results show that during the germination of pollen and the growth of pollen tube, there may be integrin-like proteins in pollen tube, consisting of alpha v and beta s integria subunits in the pollen tube of Lilium davidii Duch, which is the receptor of vitronectin-like protein.
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