| 小麦叶肉细胞原生质体中微管骨架的排列格局与[Ca^2＋]cyt的关系 |
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| 引用本文： | 刘刚,王冬梅.小麦叶肉细胞原生质体中微管骨架的排列格局与[Ca^2＋]cyt的关系[J].实验生物学报,2005,38(4):331-339. |
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| 作者姓名： | 刘刚 王冬梅 |
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| 作者单位： | 河北农业大学生命科学学院,保定071001 |
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| 基金项目： | 河北省教委博士基金资助项目（B2001211）,河北省自然科学基金重点资助项目（301124、303180、c2005000220）.教育部科学技术研究重点资助项目（03014）及植物生理学与生物化学国家重点实验室开放课题资助项目（PPB04006）. |
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| 摘 要： | 以小麦叶肉细胞原生质体为材料，通过免疫荧光标记和Ca^2＋荧光染料的装载并结合药物学试验，借助激光共聚焦扫描显微镜观察，探讨微管骨架和Ca^2＋之间的内在联系。试验结果表明，Ca^2＋]cyt的升高能够诱发微管骨架的解聚；而微管骨架的解聚也会促使胞外Ca^2＋内流，进而造成Ca^2＋]cyt的升高。
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| 关 键 词： | 小麦 叶肉细胞 原生质体 微管骨架 排列格局 钙离子 激光共聚焦扫描显微镜 叶锈病 防卫反应 |
| 收稿时间： | 2004-12-25 |
| 修稿时间： | 2004-12-25 |
Relationship of microtubule and [Ca2+]cyt in wheat mesophyll protoplast] |
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| Liu Gang;Wang DongMei.Relationship of microtubule and [Ca2+]cyt in wheat mesophyll protoplast][J].Acta Biologiae Experimentalis Sinica,2005,38(4):331-339. |
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| Authors: | Liu Gang;Wang DongMei |
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| Institution: | College of life science, Agricultural University of Hebei, Baoding 071001. |
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| Abstract: | By using immunofluorescence labeling technique and incubating the Fluo-3AM of Ca2+ fluorescein, wheat (Triticum aestivum) cultivars Lovrin 10 was selected as mesophyll protoplast material in this paper for studying the relationship between the pattern of microtubule and Ca2+]cyt. Laser scanning confocal microscopy(LSCM) was used and pharmacologic examination was set. The results indicated there was a connection between calcium signaling and microtubule organization in wheat mesophyll protoplast. When the cytosolic calcium concentration distinctly ascends, the pattern of microtubule showed depolymerization; Depolymerization of microtubule could enhance extracellar calcium afflux. |
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