| 基于Flp重组酶介导的盒式交换HPRT位点定点转基因研究 |
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| 引用本文： | 郑敬民,贺艳,等.基于Flp重组酶介导的盒式交换HPRT位点定点转基因研究[J].实验生物学报,2001,34(4):279-282. |
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| 作者姓名： | 郑敬民 贺艳 |
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| 摘 要： |
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| 关 键 词： | Flp重组酶 介导 盒式交换HPRT位点 定点转基因 |
The study of a HPRT locus-specific transgenic method based on FLP recombinase mediated cassette exchange] |
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| J M Zheng,Y He,J L Fu.The study of a HPRT locus-specific transgenic method based on FLP recombinase mediated cassette exchange][J].Acta Biologiae Experimentalis Sinica,2001,34(4):279-282. |
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| Authors: | J M Zheng Y He J L Fu |
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| Institution: | Department of Medical Genetics, Second Military Medical University, Shanghai 200433, China. |
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| Abstract: | Exchange plasmid pF-HPRT-F3 and Flp expression plasmid pCMV-Flp were constructed and then introduced using electroporation system into F18 ES cell line, which have an exchange cassette F-Neo-F3 at HPRT locus. After HAT selection, HAT resistant clones were obtained. Then G418 sensitivity test and Southern blotting were carried out to screen RMCE recombinants. The results indicated that RMCE had taken place in three of 12 HAT resistant clones. The frequency is 25%. The result demonstrates that it is realizable to introduce transgene to HPRT locus by using Flp recombinase mediated cassette exchange reaction. |
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