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甘丙肽及其受体在嗅成鞘细胞中的表达及对细胞增殖的影响
引用本文:夏趁意,戚艳婷,张烨,袁崇刚.甘丙肽及其受体在嗅成鞘细胞中的表达及对细胞增殖的影响[J].实验生物学报,2005,38(2):98-104.
作者姓名:夏趁意  戚艳婷  张烨  袁崇刚
摘    要:本实验从新生大鼠嗅球中分离出嗅成鞘细胞,进行体外培养。运用RT—PCR方法检测甘丙肽及其受体在体外培养的嗅成鞘细胞中的表达;运用MTT法检测甘丙肽及其受体激动剂、拮抗剂对嗅成鞘细胞增殖的影响。结果显示:嗅成鞘细胞表达甘丙肽(GAL)及其受体GalR2,而不表达其他两种受体GalRl和GalR3;甘丙肽及两种受体激动剂GALl-11和GAL2-11能够明显地抑制体外培养的嗅成鞘细胞的增殖,这一效应可被非特异性甘丙肽受体拮抗剂M35所阻断。

关 键 词:细胞增殖  甘丙肽  受体激动剂  体外培养  PCR方法  受体拮抗剂  新生大鼠  MTT法  细胞表达  非特异性  检测  嗅球

The expression of galanin and galanin receptors in olfactory ensheathing cells and effects of galanin on cells proliferation]
Chen Yi Xia,Yan Ting Qi,Ye Zhang,Chong Gang Yuan.The expression of galanin and galanin receptors in olfactory ensheathing cells and effects of galanin on cells proliferation][J].Acta Biologiae Experimentalis Sinica,2005,38(2):98-104.
Authors:Chen Yi Xia  Yan Ting Qi  Ye Zhang  Chong Gang Yuan
Institution:School of Life Science of East China Normal University, Shanghai 200062, China.
Abstract:Olfactory ensheathing cells (OECs) were isolated from newborn rat olfactory bulb and cultured in vitro. RT-PCR was used to determine the expression of galanin and it's receptors in these cells. MTT analysis was used to detect the effects of galanin and agonist, antagonist of galanin receptors on the proliferation of OECs. Results show that OECs express mRNAs for galanin and GalR2 but not for two another receptors, GalR1 and GalR3. In addition, galanin and two receptor agonists, GAL1-11 and GAL2-11, can significantly inhibit the proliferation of OECs. But the influence can be blocked by M35, nonspecific antagonist of galanin receptors.
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