一种简单通用的鸟类性别分子鉴定技术（简报）

我国幅员辽阔，生物类型多种多样，是世界上拥有鸟类种类最多的国家之一。截至1999年底，已知有鸟类1253种948亚种，隶属于21目83科，其中有多种为我国特有或珍稀濒危的鸟类。近年来由于环境污染、植被破坏及非法捕猎等种种原因，许多鸟类尤其是珍稀鸟类正逐步濒临灭绝。为了加紧

A simple and universal method for molecular sexing of birds]

It is difficult to sex monomorphic or juvenile birds only according to their appearance or by simple surgical techniques. However, the sex of individual bird has to be identified during breeding. Recently, a sex-linked gene, CHD1, has been discovered in non-ratite birds. Using highly conserved primers flanking the intron of CHD1 and PCR amplification, females are characterized by diaplaying two fragments(CHD1W and CHD1Z), while males only show one fragment(CHD1Z) clearly different in size from the female-specific CHD1W fragment. PCR-based molecular sexing method has been widely used due to its many merits over other techniques. In this work, we successfully sexed 86 bird from 9 species using this technique. All of these birds are listed here as the national first-grade wildlife of China for protection. Our results benefit greatly on breeding rare monomorphic birds and preventing them from distinction.