| 人胰岛素A,B链基因的合成,克隆及其在大肠杆菌中的表达 |
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| 引用本文： | 郭礼和,汪恩璧.人胰岛素A,B链基因的合成,克隆及其在大肠杆菌中的表达[J].实验生物学报,1992,25(3):283-288. |
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| 作者姓名： | 郭礼和 汪恩璧 |
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| 摘 要： |
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| 关 键 词： | 人胰岛素 基因合成 克隆 表达 |
Synthesis cloning and expressions in E coli of human insulin A and B chain genes] |
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| L H Guo,E B Wang,X Y Zhang,L H Zhu,Y J Yu,Y K Wang,G X Luo,M Y Zhou.Synthesis cloning and expressions in E coli of human insulin A and B chain genes][J].Acta Biologiae Experimentalis Sinica,1992,25(3):283-288. |
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| Authors: | L H Guo E B Wang X Y Zhang L H Zhu Y J Yu Y K Wang G X Luo M Y Zhou |
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| Institution: | Shanghai Institute of Cell Biology, Academia Sinica. |
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| Abstract: | Human insulin A and B chain genes were designed and synthesized by using a rapid and simple method. The synthesized A and B chain genes were cloned separately. The expression (plasmids) pWR 590-HIA and pWR 590-HIB were constructed, and the two plasmids can direct the synthesis of the approximately 590 amino acid-long truncated beta-galactosidases fused to human insulin A or B chains. The fused A or B chain proteins were isolated from the fermented cells and cleaved with BrCN. The resulting mixtures were sulfonated and the sulfonated A and B chains were purified. Human insulin was obtained by using an A and B chain combination method. |
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