| 兔抗人NESTIN抗血清的制备与鉴定（简报） |
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| 引用本文： | 刘磊,林万敏,林隽姬,刘璇,郭兰萍,徐平.兔抗人NESTIN抗血清的制备与鉴定（简报）[J].实验生物学报,2003,36(2):163-166. |
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| 作者姓名： | 刘磊 林万敏 林隽姬 刘璇 郭兰萍 徐平 |
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| 摘 要： |
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| 关 键 词： | 兔抗人NESTIN抗血清 GST融合蛋白 制备 鉴定 巢蛋白 免疫学检测 Western－blot 免疫印迹 免疫细胞化学 |
Preparation and identification of rabbit-anti-human nestin antiserum] |
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| Lei Liu,Wan Min Lin,Jun Ji Lin,Xuan Liu,Lan Ping Guo,Ping Xu.Preparation and identification of rabbit-anti-human nestin antiserum][J].Acta Biologiae Experimentalis Sinica,2003,36(2):163-166. |
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| Authors: | Lei Liu Wan Min Lin Jun Ji Lin Xuan Liu Lan Ping Guo Ping Xu |
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| Institution: | Laboratory of Genomic Physiology, Brain Research Center, LiRen Laboratory, Fudan University, Shanghai 200433. |
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| Abstract: | Nestin belongs to the class VI intermediate filament family and it is a marker for neural progenitor cells. In this work, the 3'-terminal coding sequence(396 bp) of human nestin gene was cloned into pGEX-3X plasmid and introduced into BL21 E. coli cells. The GST-nestin protein was purified with an affinity column. Anti-human nestin antiserum was raised by immunizing a rabbit with the fusion protein. The high specificity of the antibody against human nestin was confirmed by western-blot and immunocytochemistry analysis. |
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