| 端粒酶RNA组分的反义寡核苷酸对BEL—7404人肝癌细胞端粒酶活性的抑制和细胞凋亡的诱导 |
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| 引用本文： | 张如刚,王兴旺,等.端粒酶RNA组分的反义寡核苷酸对BEL—7404人肝癌细胞端粒酶活性的抑制和细胞凋亡的诱导[J].实验生物学报,2001,34(3):213-218. |
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| 作者姓名： | 张如刚 王兴旺 |
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| 作者单位： | [1]中国科学院上海生命科学研究院生物化学和细胞生物学研究所，上海200031 [2]中国科学院上海生命科学研究院生物化 |
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| 摘 要： |
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| 关 键 词： | 端粒酶 RNA组分 反义寡核苷酸 肝癌细胞凋亡 酶活性 |
Telomerase activity inhibition and apoptosis induction of BEL-7404 human hepatoma cells by antisense oligonucleotides to telomerase RNA component] |
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| R G Zhang,X W Wang,H Xie.Telomerase activity inhibition and apoptosis induction of BEL-7404 human hepatoma cells by antisense oligonucleotides to telomerase RNA component][J].Acta Biologiae Experimentalis Sinica,2001,34(3):213-218. |
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| Authors: | R G Zhang X W Wang H Xie |
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| Institution: | Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China. |
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| Abstract: | Telomerase activity was detected in all of four human hepatoma cells but absent in normal liver tissue. Telomerase activity of BEL-7404 human hepatoma cells was inhibited effectively by antisense oligonucleotide to telomerase RNA component at final concentration of 1 mumol/L, whereas sense and missense oligonucleotides have no effects on its activity. The inhibition of telomerase activity was weakened, as the concentration of antisense oligonucleotide decreased. Treating BEL-7404 human hepatoma cells 96 hours continuously by the antisense oligomers at final concentration of 5 mumol/L, the morphology of treated cells changed and apoptosis percent of the cells increased markedly. |
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