| 共聚焦显微技术研究ABA诱导蚕豆气孔保卫细胞H2O2的产生（简报） |
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| 引用本文： | 张骁,张霖,等.共聚焦显微技术研究ABA诱导蚕豆气孔保卫细胞H2O2的产生（简报）[J].实验生物学报,2001,34(1):71-76. |
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| 作者姓名： | 张骁 张霖 |
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| 摘 要： |
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| 关 键 词： | 蚕豆 气孔 保卫细胞 过氧化氢 ABA诱导 |
Studies on ABA-induced H2O2 in Vicia guard cells by means of confocal laser scanning microscopy] |
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| X Zhang,L Zhang,G Y An,J F Gao,C P Song.Studies on ABA-induced H2O2 in Vicia guard cells by means of confocal laser scanning microscopy][J].Acta Biologiae Experimentalis Sinica,2001,34(1):71-76. |
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| Authors: | X Zhang L Zhang G Y An J F Gao C P Song |
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| Institution: | College of Life Sciences, Northwest Sci-Tech University of Agriculture and Forestry, Yangling 712100. |
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| Abstract: | The methods of confocal laser scanning microscopy (CLSM) and microinjection were used to study ABA-induced H2O2 in guard cells (Vicia faba), which were labeled with H2O2 specific probe-2, 7-dichlorofluorescin diacetate(H2DCFDA). The results indicated 100 U/mL catalase (CAT) could inhibit partly stomatal closure induced by ABA. 10(-3) mmol/L ABA could significantly induce H2O2 production in chloroplast in guard cells of Vicia faba following microinjection, and 100 U/mL CAT could partly abolish the effects following simultaneous microinjection of ABA and CAT. These suggest that H2O2 is possibly involved in ABA signaling leading to stomatal closure. |
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