| 染色体端粒DNA与核骨架的结合关系（简报） |
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| 引用本文： | 汪国顺.染色体端粒DNA与核骨架的结合关系（简报）[J].实验生物学报,1992,25(2):185-189. |
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| 作者姓名： | 汪国顺 |
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| 摘 要： |
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| 关 键 词： | 细胞核骨架 端粒 脱氧核糖核酸 |
Association of chromosomal telomere DNA with nuclear matrix in HeLa cells] |
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| G S Wang,W J Pan,Z H Zhai.Association of chromosomal telomere DNA with nuclear matrix in HeLa cells][J].Acta Biologiae Experimentalis Sinica,1992,25(2):185-189. |
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| Authors: | G S Wang W J Pan Z H Zhai |
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| Institution: | Department of Biology, Peking University, Beijing, P. R. China. |
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| Abstract: | Nuclear matrix from HeLa cells was gently extracted with a high salt solution and treated with DNase I. DNA that remained associated with the nuclear matrix (N. M. DNA) and DNA fragments released into the supernatant (SN.DNA) were isolated respectively and dot hybridized to human telomere sequence (AGGGTT/TCCCAA)40 probe. As the time of DNase I treatment was extended, the amount of N. M. DNA decreased while the concentration of telomere sequence in N.M. DNA proportionally increased. These preliminary results suggest that the telomere sequence is tightly bound to nuclear matrix in HeLa cells. |
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