| 新型冠状病毒上海株(nCoV-SH01)的分离和鉴定 |
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| 引用本文： | 张荣,易志刚,王玉燕,滕峥,徐巍,宋武慧,蔡霞,孙志平,谷陈建,周艳秋,陈洪友,叶荣,韩文东,朱云凯,冯飞,房方皓,李崇山,张曦,瞿涤,付晨,谢幼华,袁正宏.新型冠状病毒上海株(nCoV-SH01)的分离和鉴定[J].微生物与感染,2020,15(1):16-21. |
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| 作者姓名： | 张荣 易志刚 王玉燕 滕峥 徐巍 宋武慧 蔡霞 孙志平 谷陈建 周艳秋 陈洪友 叶荣 韩文东 朱云凯 冯飞 房方皓 李崇山 张曦 瞿涤 付晨 谢幼华 袁正宏 |
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| 作者单位： | 1. 复旦大学上海医学院基础医学院病原生物学系，教育部、卫健委、医科院医学分子病毒学重点实验室，上海 200032; 2. 上海市疾病预防控制中心，上海 200336 |
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| 基金项目： | 复旦大学新型冠状病毒肺炎防治研究专项课题(新型冠状病毒分离鉴定和小鼠模型研究);“十三五”国家科技重大专项(2018ZX10734401、2019ZX09721001)。 |
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| 摘 要： | 新型冠状病毒肺炎(coronavirus disease 2019，COVID-19)疫情对人类生命健康构成极大威胁。病毒的分离是构建新型冠状病毒(2019 novel coronavirus, 2019-nCoV)细胞感染模型和动物感染模型的基础。本研究利用冠状病毒易感的Vero E6细胞，从1例上海感染者的咽拭子中分离到一株2019-nCoV，命名为nCoV-SH01。对该毒株全基因组采用一代Sanger和二代Illumina法测序，发现该毒株与GenBank MN908947的同源性＞99.99%。免疫荧光检测显示，该毒株与COVID-19康复者的血清呈阳性反应。当nCoV-SH01感染Vero E6细胞后，导致典型的合胞体病变，细胞病变效应明显且进展迅速，提示nCoV-SH01可用于进一步建立2019-nCoV的细胞感染和动物感染模型，为开展致病性研究以及抗病毒药物和疫苗研发奠定了基础。
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| 关 键 词： | 新型冠状病毒 合胞体 Vero E6细胞 鼻/咽拭子 细胞模型 |
Isolation of a 2019 novel coronavirus strain from a coronavirus disease 2019 patient in Shanghai |
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| ZHANG Rong,YI Zhigang,WANG Yuyan,TENG Zheng,XU Wei,SONG Wuhui,CAI Xia,SUN Zhiping,GU Chenjian,ZHOU Yanqiu,CHEN Hongyou,YE Rong,HAN Wendong,ZHU Yunkai,FENG Fei,FANG Fanghao,LI Chongshan,ZHANG Xi,QU Di,FU Chen,XIE Youhua,YUAN Zhenghong.Isolation of a 2019 novel coronavirus strain from a coronavirus disease 2019 patient in Shanghai[J].Journal of Microbes and Infection,2020,15(1):16-21. |
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| Authors: | ZHANG Rong YI Zhigang WANG Yuyan TENG Zheng XU Wei SONG Wuhui CAI Xia SUN Zhiping GU Chenjian ZHOU Yanqiu CHEN Hongyou YE Rong HAN Wendong ZHU Yunkai FENG Fei FANG Fanghao LI Chongshan ZHANG Xi QU Di FU Chen XIE Youhua YUAN Zhenghong |
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| Institution: | 1. Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), and Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032, China; 2. Shanghai Center for Disease Control and Prevention, Shanghai 200336, China |
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| Abstract: | The spread of 2019 novel coronavirus(2019-nCoV)poses a serious threat to human health.We report here the isolation of a 2019-nCoV strain named nCoV-SH01 from the throat swab from a COVID-19 patient in Shanghai.The isolate grows well on Vero E6 cells with typical syncytial cytopathic effects.The nCoV-SH01 genome has been sequenced using sanger sequencing and Illumina sequencing and analyzed,which shares more than 99.99%identity to a reference sequence(GenBank MN9886682019-nCoV Wuhan-Hu-1 isolate)with a difference of only three nucleotides.Immunofluorescence results showed that nCoV-SH01-infected cells reacted with the serum of a convalescent COVID-19 patient,suggesting virus replication in cultured cells.The isolation of nCoV-SH01 provides the basis on which cell and animal infection models would be established to facilitate the study of 2019-nCoV pathogenicity and the development of antiviral drugs and vaccines. |
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| Keywords: | 2019 novel coronavirus Syncytium Vero E6 cell Nasal/throat swab Cell model |
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