溴氰菊酯对不同品系家蝇脑突触体膜蛋白磷酸化及ATP酶活性的影响

以敏感品系家蝇和溴氰菊酯抗性品系家蝇(Musca domestica L.)为材料，研究和比较了神经毒剂溴氰菊酯对其脑突触体蛋白磷酸化作用的影响。结果表明，浓度为10^-5 mol/L溴氰菊酯抑制了敏感品系家蝇脑突触体蛋白磷酸化作用，而对抗性品系家蝇脑突触体蛋白磷酸化作用无明显影响。若反应体系中加入2.5×10^-6 mol/L的cAMP显著激活了敏感品系家蝇脑突触体蛋白磷酸化水平，但是当0.6 mmol/Lca²⁺或0.6 mmol/L Ca²⁺加10^-5 mol/L钙调蛋白时明显增强了抗性品系家蝇脑突触体蛋白磷酸化水平，甚至超过了其对敏感品系的作用水平。此外，还发现不同浓度的溴氰菊酯可抑制突触膜上的Na/K-ATP酶和Ca-ATP酶活力，浓度越高抑制作用也越大，并且敏感品系家蝇对溴氰菊酯的敏感度要高于抗性品系。

Inhibition of protein phosphorylation and ATPase activity by deltamethrin in housefly brain synapto- somes

Deltamethrin was found to affect ATPase activity and protein phosphorylation of housefly brain synaptosome in vitro. Both Na, K-ATPase and Ca-ATPase activities can be inhibited by various concentrations of deltamethrin (10^-9～10^-4mol/L). However, the inhibition was higher in susceptible housefly than in deltamethrin resistant strain. In intact susceptible housefly brain synaptosome the most significant result was inhibition of endogenous protein phosphorylation by 10^-5 mol/L of deltamethrin. Such effect in deltamethrin resistant housefly was suppressed by adding the exogenous Ca²⁺ or Ca²⁺ and calmodolin to the reaction system.Cyclic AMP stimulated the overall phosphorylation level,particularly 48 kD protein in susceptible housefly. The 48 kD protein,isoelectric point pI 5.2,may be the brain-specific B-50 protein in housefly.All these results indicated that: (1)Deltamethrin has the property to affect ATPase activity and protein phosphorylation. (2) Deltamethrin might limits calcium transport which connects with the protein phosphorylation.