甜菜夜蛾抗高效氯氟氰菊酯近等基因系的构建

将甜菜夜蛾Spodoptera exigua抗性种群雄成虫与敏感种群雌成虫杂交，杂交后代再自交后，用高效氯氟氰菊酯杀死敏感和部分杂合个体的剂量(50 μg/mL)处理4龄幼虫，对1天后存活的幼虫再用250 μg/mL汰选，将存活幼虫发育成的雄成虫与敏感种群的雌成虫回交，再经自交和选育，如此循环6次，获得了甜菜夜蛾抗高效氯氟氰菊酯近等基因系。对该基因系构建过程中各代幼虫的抗性水平和相关生物学特性进行了监测和比较，结果表明获得的抗高效氯氟氰菊酯近等基因系抗性种群的抗药性仍保持较高水平；酯酶同工酶的电泳分析表明抗高效氯氟氰菊酯近等基因系抗性种群与敏感种群图谱相近，而与亲本抗性种群之间存在明显差异。

Establishment of the near isogenic line strain resistant to lambda-cyhalothrin in Spodoptera exigua ( Hübner ) (Lepidoptera:Noctuidae)

The lambda-cyhalothrin resistant males and susceptible females in Spodoptera exigua were crossed in the laboratory. The 4th-instar larvae derived from the F₂ inbred progeny were treated with the dose of 50 μg/mL of lambda-cyhalothrin, and survivors were taken into the second selection at the dose of 250 μg/mL in the following day. The survival adults (♂) after the second selection were backcrossed with the susceptible adults (♀)，and their inbred progeny was selected with a similar method until the near isogenic line (NIL) strain resistant to lambda-cyhalothrin were established after the process was repeated 6 times. During the establishment process of the NIL strain, the resistance level and biological parameters in every generation were assayed. The results indicated that resistance level to lambda-cyhalothrin of NIL-RR (resistant strain) had no significant decrease compared to the parent resistant strain. Esterase analysis to the 4th instar larvae of the susceptible, resistant and NIL-RR strains showed that the pattern of esterase band of NIL-RR was similar to that of parent susceptible strain, but significantly different with the parent resistant.