绿原酸提高舞毒蛾核型多角体病毒(LdNPV)的致病力

【目的】明确绿原酸(chlorogenic acid,CA)对舞毒蛾核型多角体病毒Lymantria dispar necleopolyhydrovirus(Ld NPV)致病力的影响,为舞毒蛾的防治提供参考依据。【方法】采用食料给毒法进行生物测定,测定舞毒蛾2龄幼虫对单独Ld NPV及添加绿原酸的Ld NPV(CA+Ld NPV)的剂量及致死时间响应。【结果】CA+Ld NPV与单独Ld NPV对舞毒蛾2龄幼虫的剂量及时间响应间有显著差异,二者对舞毒蛾2龄幼虫的致死中浓度(LC50)分别为161.8 OBs/μL(95%的置信区间为105.6~235.3 OBs/μL)和264.4 OBs/μL(95%的置信区间为178.6~384.0 OBs/μL),前者对舞毒蛾的致病力较后者强。CA+Ld NPV对舞毒蛾2龄幼虫的致死中时间(LT_(50))较Ld NPV的短,当Ld NPV浓度为590 OBs/μL,CA+Ld NPV及Ld NPV对舞毒蛾2龄幼虫的LT_(50)分别为9.9 d和12.3 d;当Ld NPV浓度为5 900 OBs/μL时,则LT_(50)分别为6.9 d和8.0 d。绿原酸降低了Ld NPV对舞毒蛾幼虫的致死中浓度,缩短了致死中时间。【结论】绿原酸可提高Ld NPV对舞毒蛾2龄幼虫的致病力,其机理有待进一步研究。

Chlorogenic acid enhances the virulence of Lymantria dispar necleopolyhydrovirus (LdNPV)

Aim] To identify the effects of chlorogenic acid (CA) on the virulence of Lymantria dispar necleopolyhydrovirus (LdNPV) so as to provide information for the prevention and control of the gypsy moth,L.dispar.Methods] CA + LdNPV and LdNPV were bioassayed using diet plus method,to determine the dose and time response of the 2nd instar larvae of L.dispar.Results] CA + LdNPV and LdNPV differed significantly in dose and time response when they were tested against the L.dispar larvae.The median lethal concentration (LC50) value of CA + LdNPV to the 2nd instar larvae was 161.8 OBs/μL (the 95％ confidence limit was 105.6-235.3OBs/μL),and that of LdNPV was 264.4OBs/ μL (the 95％ confidence limit was 178.6-384.0OBs/μL).CA + LdNPV was more virulent than LdNPV against L.dispar larvae.The median lethal time (LT50) value of CA + LdNPV was shorter than that of LdNPV.The LT50 values of CA + LdNPV and LdNPV were 9.9 and 12.3 days,respectively,at the concentration of 590 OBs/μL of LdNPV,and 6.9 and 8.0 days,respectively,at the concentration of 5 900 OBs/μL.The CA lowered the LC50 value and shortened the LT50value of LdNPV against the 2nd instar larvae of L.dispar.Conclusion] Chlorogenic acid enhances the virulence of LdNPV against the 2nd instar larvae of L.dispar,and its action mechanism needs further study.