高温处理后褐飞虱体内共生酵母菌和氨基酸需求的变化

对卵期高温（35℃，72 h）处理后褐飞虱Nilaparvata lugens体内共生酵母菌及该虫氨基酸需求的变化进行了研究，结果表明：1）卵期高温处理后，若虫期体内的共生酵母菌数量明显低于正常对照试虫；2）将若虫饲养于缺少单种氨基酸的22种全纯饲料上，以若虫羽化率为指标，发现高温处理试虫对组氨酸、赖氨酸、亮氨酸、异亮氨酸、苏氨酸、缬氨酸、苯丙氨酸和色氨酸等8种必需氨基酸的需求明显增加。由此推论，褐飞虱对上述8种必需氨基酸的需求与共生酵母菌有关。

The effects of high temperature on both yeast-like symbionts and amino acid requirements of Nilaparvata lugens

The brown planthopper, Nilaparvata lugens, was subject to a 35℃ treatment for 72 h at the egg stage, and the development of yeast-like symbionts and amino acid requirements of both heat-treated and untreated N. lugens were studied. The results indicated that heat-treated N. lugens harbored substantially fewer symbionts at the nymphal stage as compared with untreated one. By rearing on 22 chemically defined diets, with single amino acid deleted for each diet, only the requirements of heat-treated N. lugens for 8 essential amino acids, i.e. His, Lys, Leu, Ile, Thr, Val, Phe, Try, increased obviously with the rate of adult emergence as indicator. It was proposed that the yeast-like symbionts be closely related to the requirements of above 8 essential amino acids for N. lugens.