棉铃虫抗药性监测方法——浸叶法敏感毒力基线的建立及其应用

通过室内饲养的2个棉铃虫敏感品系，用浸叶法建立了11种常用药剂 (氯氰菊酯、溴氰菊酯、功夫菊酯、氰戊菊酯、久效磷、辛硫磷、甲基对硫磷、毒死蜱、灭多威、硫双灭多威、硫丹) 的敏感毒力基线，确定了它们的LC₅₀值和区分剂量。并用浸叶法监测了江苏、山东、河南、安徽4省棉田2代棉铃虫对氯氰菊酯、久效磷、灭多威和辛硫磷的抗性，结果表明用区分剂量监测抗性个体频率既快速简便，又适宜于进行早期抗性检测，因此这4种药剂的区分剂量可以推广应用于棉铃虫田间抗药性监测。

Susceptible toxicity baseline and its application in resistance monitoring of Helicoverpa armigera with leaf dipping method

Susceptible toxicity baselines of Helicoverpa armigera to eleven conventional insecticides(cypermethrin, deltamethrin, cyhalothrin, fenvalerate, monocrotophos, phoxim, methyl parathion, chlorpyrifos, methomyl, thiodicarb and endosulfan)were established with leaf dipping method, and then LC 50 and discriminating dose were determined respectively. Resistance to cypermethrin, monocrotophos, methomyl and phoxim in H. armigera from four provinces(Jiangsu, Shandong, Henan and Anhui)was monitored by leaf dipping method and the baseline data were established. The results indicated that using discriminating dose to monitor resistant individual frequency was more rapid and simple than comparing resistance ratio at LC 50 . Discriminating dose technique is suitable for early resistance detection and discriminating doses of the four insecticides mentioned above can be extended in resistance monitoring program of H. armigera in China.