环丙氨嗪对鸡粪中亮斑扁角水虻生长的影响及添加活性炭对环丙氨嗪的解毒作用

【目的】研究环丙氨嗪对鸡粪中亮斑扁角水虻Hermetia illucens幼虫生长发育的影响及添加活性炭对环丙氨嗪的解毒作用。【方法】使用含不同浓度(0, 0.1, 0.25, 0.5, 1, 2.5, 5, 10和15 mg/kg)环丙氨嗪的鸡粪分别饲养3, 4和5龄亮斑扁角水虻幼虫，统计幼虫的生长发育(体重和死亡率)变化；在鸡粪中添加不同浓度(0, 10, 30, 50, 100和150 g/kg)活性炭饲养亮斑扁角水虻3龄幼虫，测定活性炭对其生长发育的影响以及对环丙氨嗪的解毒作用。【结果】在用含一定浓度环丙氨嗪的鸡粪饲养后，亮斑扁角水虻3, 4和5龄幼虫均表现出体节伸长，停止进食，直至死亡。鸡粪中环丙氨嗪对亮斑扁角水虻3, 4和5龄幼虫的致死中浓度(medium lethal concentration, LC₅₀)值分别为0.18, 1.39和6.45 mg/kg。在鸡粪中添加量为30~100 g/kg的活性炭可以显著促进亮斑扁角水虻3龄幼虫的生长发育，并且能够解除环丙 氨嗪对3龄幼虫的毒性，95%有效剂量(95% effective dose, ED₉₅)值为51.83 g/kg。【结论】随着亮斑扁角水虻幼虫龄期增长，其对鸡粪中环丙氨嗪的敏感性逐渐降低。活性炭可用于解除鸡粪中环丙氨嗪对亮斑扁角水虻幼虫的毒性，为生产上应用亮斑扁角水虻处理含环丙氨嗪的鸡粪提供解决方案。

Effects of cyromazine on the growth ofHermetia illucens(Diptera: Stratiomyidae) inchicken manure and the detoxification of cyromazine by added activated carbon

【Aim】 This study aims to study the effects of cyromazine on the growth anddevelopment of the black soldier fly (Hermetia illucens) larvae in chicken manure and thedetoxification of cyromazine by the added activated carbon. 【Methods】 The 3rd, 4th and 5th instar larvae of H. illucens were reared in the chicken manure containing differentconcentrations (0, 0.1, 0.25, 0.5, 1, 2.5, 5, 10 and 15 mg/kg) of cyromazine, and thechanges in the growth and development (body weight and mortality) of the larvae weremeasured. Reared in the chicken manure added with activated carbon at differentconcentrations (0, 10, 30, 50, 100, and 150 g/kg), the effects of activated carbon on thegrowth and development of the 3rd instar larvae of H. illucens and its detoxification effect on cyromazine were assayed. 【Results】 Reared in the chicken manure containingcertain concentration of cyromazine, the 3rd, 4th and 5th instar larvae of H. illucensshowed such symptoms as elongation of somites, cessation of feeding and even death. Themedium lethal concentration (LC₅₀) values of cyromazine to the 3rd, 4th and 5th instarlarvae of H. illucens were 0.18, 1.39 and 6.45 mg/kg, respectively. Activated carbon atthe concentrations of 30-100 g/kg in chicken manure significantly promoted the growth anddevelopment of the 3rd instar larvae of H. illucens and detoxified the toxicity ofcyromazine to the 3rd instar larvae with the 95% effective dose (ED₉₅) value of 51.83 g/kg.【Conclusion】 The sensitivity of H. illucens larvae to cyromazine in chicken manuregradually decreases with larval instar. Activated carbon can be used to detoxify thetoxicity of cyromazine in chicken manure to H. illucens larvae, providing a solution forapplication of H. illucens to process chicken manure containing cyromazine.